Doctorate Program in Biomedical Sciences, University Center for Health Sciences, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Department of Clinics, University Center of Los Altos, Tepatitlan de Morelos, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Oncol Rep. 2018 Mar;39(3):1253-1260. doi: 10.3892/or.2018.6222. Epub 2018 Jan 19.
Prolactin (PRL) is associated with different types of cancer, such as cervical cancer. Recombinant PRL has antiapoptotic effect on cervical cancer cells, and it can also induce cytokine production on macrophages. A 60 kDa variant of PRL is produced by cervical cancer cells. The aim of the present study was to evaluate this variant's bioactivity, to test its effect on cervical cancer cell apoptosis, and to assess its ability to induce cytokine production on THP-1 macrophages. First, 60 kDa PRL was isolated and used to stimulate Nb2 cells. Later, apoptosis was measured after exposure to 60 kDa PRL. Finally, cytokines were measured on THP-1 stimulated supernatants. Our results show that 60 kDa PRL increased Nb2 cell proliferation. Apoptosis was decreased after stimuli with 60 kDa PRL in cervical cancer cells. IL-1β and TNF-α are produced by THP-1 macrophages after stimuli. These results suggest that 60 kDa PRL produced by cervical cancer cells is able to reduce apoptosis in HeLa, SiHa and C-33A cells and induce IL-1β and TNF-α production by THP-1 macrophages.
催乳素 (PRL) 与不同类型的癌症有关，如宫颈癌。重组 PRL 对宫颈癌细胞具有抗凋亡作用，还可以诱导巨噬细胞产生细胞因子。宫颈癌细胞产生一种 60kDa 的 PRL 变体。本研究旨在评估该变体的生物活性，检验其对宫颈癌细胞凋亡的影响，并评估其诱导 THP-1 巨噬细胞产生细胞因子的能力。首先，分离出 60kDa PRL 并用其刺激 Nb2 细胞。然后，在暴露于 60kDa PRL 后测量细胞凋亡。最后，测量 THP-1 刺激上清液中的细胞因子。我们的结果表明，60kDa PRL 增加了 Nb2 细胞的增殖。60kDa PRL 刺激宫颈癌细胞后，细胞凋亡减少。THP-1 巨噬细胞产生 IL-1β 和 TNF-α。这些结果表明，宫颈癌细胞产生的 60kDa PRL 能够减少 HeLa、SiHa 和 C-33A 细胞的凋亡，并诱导 THP-1 巨噬细胞产生 IL-1β 和 TNF-α。
