Zsigmond E, Clandinin M T
Int J Biochem. 1986;18(6):505-11. doi: 10.1016/0020-711x(86)90160-6.
Weanling rats were fed diets differing in fatty acid composition to determine if changes induced in cardiac mitochondrial membrane structural components alter the sensitivity of mitochondrial ATPase to inhibition by oligomycin and stimulation by 2,4-dinitrophenol. Mitochondrial ATPase assayed in situ within the mitochodrial membrane isolated from animals fed diets higher in fatty acids of longer chain length, exhibited greater oligomycin sensitivity and lower 2,4-dinitrophenol-induced stimulation. Concomitant diet-induced changes occur in the fatty acid composition of phosphatidylcholine, phosphatidylethanolamine and cardiolipin, increasing overall length of fatty-acyl tails in the membrane phospholipids. Diet fat mediated alterations in oligomycin sensitivity of mitochondrial ATPase and membrane fatty acid chain length suggest that in vivo changes in thickness of the lipid bilayer may alter mitochondrial ATPase functions. The present study extends the concept that dietary fat affects mitochondrial membrane structure and function by demonstrating that the membrane-dependent sensitivity of mitochondrial ATPase to inhibitors and stimulators may be modulated by dietary fat.
给断奶大鼠喂食脂肪酸组成不同的日粮，以确定心脏线粒体膜结构成分的变化是否会改变线粒体ATP酶对寡霉素抑制和2,4-二硝基苯酚刺激的敏感性。从喂食长链脂肪酸含量较高日粮的动物分离出的线粒体膜中，原位测定的线粒体ATP酶对寡霉素更敏感，对2,4-二硝基苯酚诱导的刺激更低。日粮诱导的同时变化发生在磷脂酰胆碱、磷脂酰乙醇胺和心磷脂的脂肪酸组成中，增加了膜磷脂中脂肪酰尾的总长度。日粮脂肪介导的线粒体ATP酶对寡霉素敏感性和膜脂肪酸链长度的改变表明，体内脂质双层厚度的变化可能会改变线粒体ATP酶的功能。本研究通过证明线粒体ATP酶对抑制剂和刺激剂的膜依赖性敏感性可能受日粮脂肪调节，扩展了日粮脂肪影响线粒体膜结构和功能的概念。
