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一些硬骨鱼中粉色肌纤维的组织化学和免疫组织化学特征

Histochemical and immunohistochemical profile of pink muscle fibres in some teleosts.

作者信息

Mascarello F, Romanello M G, Scapolo P A

出版信息

Histochemistry. 1986;84(3):251-5. doi: 10.1007/BF00495791.

DOI:10.1007/BF00495791
PMID:2940205
Abstract

The pink muscle of several Teleosts was examined immunohistochemically using antisera specific for the myosins of red and white muscle, and histochemically using various methods for demonstrating myosin ATPase (mATPase) activity. In the catfish the pink muscle consists of 2 different layers of fibres. The superficial layer has a low mATPase activity after both acid and alkali pre-incubation, whereas the deeper layer has a high mATPase activity after acid and alkali pre-incubation, being more resistent to these conditions even than is the white muscle. In the trout the pink muscle is composed of fibres with the same mATPase activity as in the superficial pink muscle of the catfish, whereas in the rock goby, goldfish, mullet and guppy the pink muscle is like the deep pink layer of the catfish. Immunohistochemically the fibres of the pink muscle behave like the white muscle fibres except in the guppy and rock goby in which at the level of the lateral line there occurs a transition zone between red and pink fibres. The fibres of this region react with both anti-fast and (to a lesser extent) anti-slow myosin antisera, and have a mATPase activity which, going from the superficial to the deeper fibres, gradually loses the red muscle characteristics to acquire those of the main pink muscle layer.

摘要

使用针对红肌和白肌肌球蛋白的抗血清对几种硬骨鱼的粉色肌肉进行了免疫组织化学检查,并使用各种方法对肌球蛋白ATP酶(mATP酶)活性进行了组织化学检测。在鲶鱼中,粉色肌肉由两层不同的纤维组成。表层在酸和碱预孵育后mATP酶活性较低,而深层在酸和碱预孵育后mATP酶活性较高,甚至比白肌更耐这些条件。在鳟鱼中,粉色肌肉由与鲶鱼表层粉色肌肉中mATP酶活性相同的纤维组成,而在岩虾虎鱼、金鱼、鲻鱼和孔雀鱼中,粉色肌肉类似于鲶鱼的深层粉色层。免疫组织化学显示,粉色肌肉的纤维表现得与白肌纤维相似,除了在孔雀鱼和岩虾虎鱼中,在侧线水平处红肌纤维和粉色纤维之间存在一个过渡区。该区域的纤维与抗快肌和(程度较轻)抗慢肌肌球蛋白抗血清都发生反应,并且具有mATP酶活性,从表层纤维到深层纤维,逐渐失去红肌特征而获得主要粉色肌肉层的特征。

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