Yao Han-Chun, Sun Min, Yang Xiao-Feng, Zhang Zhen-Zhong, Li Hua
School of Pharmaceutical Science, Zhengzhou University, Zhengzhou 450001, China.
Department of Chemistry, Northwest University, Xi'an 710069, China.
J Pharm Anal. 2011 Feb;1(1):32-38. doi: 10.1016/S2095-1779(11)70006-5. Epub 2012 Jan 30.
The combined use of chemometrics and chemiluminescence (CL) measurements, with the aid of the stopped-flow mixing technique, developed a simple time-resolved CL method for the simultaneous determination of captopril (CPL) and hydrochlorothiazide (HCT). The stopped-flow technique in a continuous-flow system was employed in this work in order to emphasize the kinetic differences between the two analytes in cerium (IV)-rhodamine 6G CL system. After the flow was stopped, an initial rise of CL signal was observed for HCT standards, while a direct decay of CL signal was obtained for CPL standards. The mixed CL signal was monitored and recorded on the whole process of continuousflow/stopped-flow, and the obtained data were processed by the chemometric approach of artificial neural network. The relative prediction error (RPE) of CPL and HCT was 5.9% and 8.7%, respectively. The recoveries of CPL and HCT in tablets were found to fall in the range between 95% and 106%. The proposed method was successfully applied to the simultaneous determination of CPL and HCT in a compound pharmaceutical formulation.
借助停流混合技术，将化学计量学与化学发光（CL）测量相结合，开发出一种简单的时间分辨CL方法，用于同时测定卡托普利（CPL）和氢氯噻嗪（HCT）。本研究采用连续流动系统中的停流技术，以突出铈（IV）-罗丹明6G CL体系中两种分析物之间的动力学差异。停流后，观察到HCT标准品的CL信号最初上升，而CPL标准品的CL信号则直接衰减。在连续流动/停流的全过程中监测并记录混合CL信号，所得数据采用人工神经网络化学计量学方法进行处理。CPL和HCT的相对预测误差（RPE）分别为5.9%和8.7%。片剂中CPL和HCT的回收率在95%至106%之间。所提出的方法成功应用于复方药物制剂中CPL和HCT的同时测定。
