Fu Zhaofu, Huang Wanting, Li Gongke, Hu Yufei
School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
J Pharm Anal. 2017 Aug;7(4):252-257. doi: 10.1016/j.jpha.2017.05.005. Epub 2017 May 19.
A novel flow-injection chemiluminescence (FI-CL) method free of CL reagent was developed for the determination of captopril based on its enhancing effect on the CL derived from diperiodatoargentate(III)-sulfuric acid system. Compared with the conventional CL system, the CL system based on trivalent silver was characterized of good selectivity for the absence of CL reagent. The CL mechanism was discussed through CL spectra and UV-vis absorption spectra. The conditions of the FI-CL system were investigated and optimized. Under the optimal conditions, the relative CL intensity was linear with the captopril concentration in the range of 0.3-15.0 μg/mL. The detection limit for captopril was 0.05 μg/mL, and the relative standard deviation (=11) was 2.0% for 5.0 μg/mL captopril. The proposed method was applied to the analysis of captopril in tablet and human urine with the recoveries of 83.1%-112.5%, and the relative standard deviations of 0.5%-4.4%. The results obtained by the proposed method agreed well with those obtained from HPLC method. The proposed method is fast, convenient, and cost-effective for the determination of captopril in medicine and biological samples.
基于卡托普利对高碘酸银（III）-硫酸体系产生的化学发光（CL）增强作用，开发了一种无需CL试剂的新型流动注射化学发光（FI-CL）法用于测定卡托普利。与传统CL体系相比，基于三价银的CL体系因无需CL试剂而具有良好的选择性。通过CL光谱和紫外-可见吸收光谱对CL机理进行了探讨。对FI-CL体系的条件进行了研究和优化。在最佳条件下，相对CL强度与卡托普利浓度在0.3 - 15.0μg/mL范围内呈线性关系。卡托普利的检测限为0.05μg/mL，对于5.0μg/mL卡托普利，相对标准偏差（n = 11）为2.0%。该方法应用于片剂和人尿中卡托普利的分析，回收率为83.1% - 112.5%，相对标准偏差为0.5% - 4.4%。该方法所得结果与高效液相色谱法所得结果吻合良好。该方法快速、便捷且成本效益高，可用于药物和生物样品中卡托普利的测定。
