用于测定加标人血浆中依普利酮的反相高效液相色谱法的建立与验证
Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma.
作者信息
Gide Paraag, Sonawane Sandeep, Chitnis Abhishek
机构信息
MET's Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Nashik 422003, Maharashtra, India.
Department of Clinical Sciences and Administration, University of Houston, College of Pharmacy, Houston, TX 77030, USA.
出版信息
J Pharm Anal. 2012 Oct;2(5):390-393. doi: 10.1016/j.jpha.2012.04.006. Epub 2012 Apr 24.
A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column (250 mm×4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile:water (50:50, v/v) at flow rate of 1 mL/min. The calibration curve was linear in the range 100-3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor 1/.
建立了一种快速简便的高效液相色谱（HPLC）方法，采用紫外检测（241nm），用于测定加标人血浆中的依普利酮，并进行了方法验证。分析物和内标（伐地昔布）用二氯甲烷和乙醚的混合物萃取。色谱分离在HiQSil C-18HS柱（250mm×4.6mm，5μm）上进行，流动相为乙腈：水（50:50，v/v），流速为1mL/min。校准曲线在100-3200ng/mL范围内呈线性，通过使用加权因子为1/的加权最小二乘法回归将异方差性降至最低。
Zotero 插件