Broeckhoven K, Cabooter D, Desmet G
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
KU Leuven, Department of Pharmaceutical Sciences, Herestraat 49, 3000 Leuven, Belgium.
J Pharm Anal. 2013 Oct;3(5):313-323. doi: 10.1016/j.jpha.2012.12.006. Epub 2012 Dec 25.
The reintroduction of superficially porous particles has resulted in a leap forward for the separation performance in liquid chromatography. The underlying reasons for the higher efficiency of columns packed with these particles are discussed. The performance of the newly introduced 5 μm superficially porous particles is evaluated and compared to 2.7 μm superficially porous and 3.5 and 5 μm fully porous columns using typical test compounds (alkylphenones) and a relevant pharmaceutical compound (impurity of amoxicillin). The 5 μm superficially porous particles provide a superior kinetic performance compared to both the 3.5 and 5 μm fully porous particles over the entire relevant range of separation conditions. The performance of the superficially porous particles, however, appears to depend strongly on retention and analyte properties, emphasizing the importance of comparing different columns under realistic conditions (high enough ) and using the compound of interest.
表面多孔颗粒的重新引入使液相色谱的分离性能实现了飞跃。本文讨论了填充这些颗粒的色谱柱效率更高的潜在原因。使用典型测试化合物（烷基酚）和一种相关药物化合物（阿莫西林杂质）对新引入的5μm表面多孔颗粒的性能进行了评估，并与2.7μm表面多孔以及3.5μm和5μm全多孔色谱柱进行了比较。在整个相关分离条件范围内，5μm表面多孔颗粒与3.5μm和5μm全多孔颗粒相比具有更优异的动力学性能。然而，表面多孔颗粒的性能似乎强烈依赖于保留和分析物性质，这凸显了在实际条件下（足够高）比较不同色谱柱并使用目标化合物的重要性。
