Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
J Chromatogr A. 2012 Mar 9;1228:20-30. doi: 10.1016/j.chroma.2011.08.003. Epub 2011 Aug 10.
The present contribution reviews the foundations of the kinetic-plot method for the direct comparison of the kinetic performance of different chromatographic support and operating modes. The method directly uses experimental data collected for a specific sample and operating condition of one's interest, and is applicable both under isocratic- and gradient-elution conditions. Experimental proof is provided for the strong relation between the kinetic performance of a given support under isocratic and gradient conditions: a material offering superior kinetic performances under isocratic conditions will remain superior under gradient conditions and vice versa provided the comparison occurs under unbiased conditions. In addition, a review is made of the recent literature using the kinetic-plot method to compare and assess the kinetic performance of high performance HPLC columns and their operation mode.
本贡献回顾了动力学谱图法的基础，该方法可用于直接比较不同色谱支撑体和操作模式的动力学性能。该方法直接使用针对特定样品和感兴趣的操作条件收集的实验数据，并且在等度和梯度洗脱条件下均适用。实验证明了在等度和梯度条件下给定支撑体的动力学性能之间存在很强的关系：在等度条件下表现出优越动力学性能的材料在梯度条件下仍将保持优越，反之亦然，只要比较是在无偏条件下进行的。此外，还对最近使用动力学谱图法比较和评估高效 HPLC 柱及其操作模式的动力学性能的文献进行了回顾。
