Haneef Jamshed, Shaharyar Mohammad, Husain Asif, Rashid Mohd, Mishra Ravinesh, Parveen Shama, Ahmed Niyaz, Pal Manoj, Kumar Deepak
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
Department of Chemistry, Indian Institute of Technology (IIT), Haridwar, Roorkee 247667, Uttarakhand, India.
J Pharm Anal. 2013 Oct;3(5):341-348. doi: 10.1016/j.jpha.2013.03.005. Epub 2013 Apr 2.
Liquid chromatography tandem mass chromatography (LC-MS/MS) is an important hyphenated technique for quantitative analysis of drugs in biological fluids. Because of high sensitivity and selectivity, LC-MS/MS has been used for pharmacokinetic studies, metabolites identification in the plasma and urine. This manuscript gives comprehensive analytical review, focusing on chromatographic separation approaches (column packing materials, column length and mobile phase) as well as different acquisition modes (SIM, MRM) for quantitative analysis of glucocorticoids and stimulants. This review is not meant to be exhaustive but rather to provide a general overview for detection and confirmation of target drugs using LC-MS/MS and thus useful in the doping analysis, toxicological studies as well as in pharmaceutical analysis.
液相色谱串联质谱法(LC-MS/MS)是用于生物体液中药物定量分析的一种重要联用技术。由于具有高灵敏度和高选择性,LC-MS/MS已被用于药代动力学研究、血浆和尿液中代谢物的鉴定。本手稿给出了全面的分析综述,重点关注用于糖皮质激素和兴奋剂定量分析的色谱分离方法(柱填充材料、柱长和流动相)以及不同的采集模式(SIM、MRM)。本综述并非详尽无遗,而是旨在提供使用LC-MS/MS检测和确证目标药物的一般概述,从而在兴奋剂检测分析、毒理学研究以及药物分析中发挥作用。