Hwang Soyoun, West Greenlee M Heather, Balkema-Buschmann Anne, Groschup Martin H, Nicholson Eric M, Greenlee Justin J
United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, Ames, IA, United States.
Department of Biomedical Sciences, Iowa State University College of Veterinary Medicine, Ames, IA, United States.
Front Vet Sci. 2018 Jan 19;4:242. doi: 10.3389/fvets.2017.00242. eCollection 2017.
Bovine spongiform encephalopathy (BSE) belongs to a group of fatal prion diseases that result from the misfolding of the cellular prion protein (PrP) into a pathogenic form (PrP) that accumulates in the brain. assays such as serial protein misfolding amplification and real-time quaking-induced conversion (RT-QuIC) allow assessment of the conversion of PrP to PrP. RT-QuIC can be used for the detection of prions in a variety of biological tissues from humans and animals. However, there is no such comparison of RT-QuIC data between BSE positive and presymptomatic cattle. Further, the current study assesses prion distribution in multiple brain regions of clinically ill or subclinical animals. Here, we compare RT-QuIC reactions seeded with brain samples collected from experimentally inoculated cattle that were clinically ill or subclinically affected with BSE. The results demonstrate RT-QuIC seeding in various brain regions of an animal with subclinical BSE despite being determined negative by immunohistochemistry. Bioassay of the subclinical animal and RT-QuIC of brainstem from inoculated knockout () cattle were used to confirm infectivity in the subclinical animal and determine that RT-QuIC reactions were not the result of residual inoculum, respectively. These results confirm that RT-QuIC is a highly sensitive prion detection assay that can detect prions in a steer prior to the onset of clinical signs of BSE.
牛海绵状脑病（BSE）属于一组致命的朊病毒疾病，由细胞朊病毒蛋白（PrP）错误折叠成致病形式（PrP）所致，该致病形式在大脑中蓄积。诸如连续蛋白质错误折叠扩增和实时颤抖诱导转化（RT-QuIC）等检测方法可用于评估PrP向PrP的转化。RT-QuIC可用于检测人和动物各种生物组织中的朊病毒。然而，尚未对BSE阳性牛和症状前牛的RT-QuIC数据进行此类比较。此外，当前研究评估了临床患病或亚临床动物多个脑区的朊病毒分布。在此，我们比较了用从实验接种的临床患病或亚临床感染BSE的牛采集的脑样本接种的RT-QuIC反应。结果表明，尽管经免疫组织化学检测呈阴性，但亚临床BSE动物的各个脑区均出现了RT-QuIC接种。对接种的基因敲除（）牛的亚临床动物进行生物测定和脑干的RT-QuIC，分别用于确认亚临床动物的传染性并确定RT-QuIC反应不是残留接种物的结果。这些结果证实，RT-QuIC是一种高度灵敏的朊病毒检测方法，可在BSE临床症状出现之前检测出公牛体内的朊病毒。
