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噬菌体T2、T4和T6的α-葡萄糖基转移酶。它们一级结构的比较。

The alpha-glucosyltransferases of bacteriophages T2, T4 and T6. A comparison of their primary structures.

作者信息

Gram H, Rüger W

出版信息

Mol Gen Genet. 1986 Mar;202(3):467-70. doi: 10.1007/BF00333278.

DOI:10.1007/BF00333278
PMID:2940438
Abstract

With the aim of comparing the primary structures of gene products coded for by T-even bacteriophages we constructed clone libraries of the DNAs of bacteriophages T2 and T6. Using hybrid M13 phages carrying the gene for the T4-coded alpha-glucosyl transferase (alpha gt) we isolated corresponding T2 and T6 clones. The nucleotide sequences of the three alpha gt genes and the amino acid sequences derived were compared. The differences between the genes and their products are discussed in terms of structure, function and evolutionary aspects.

摘要

为了比较T偶数噬菌体编码的基因产物的一级结构，我们构建了噬菌体T2和T6 DNA的克隆文库。利用携带T4编码的α-葡萄糖基转移酶（αgt）基因的杂种M13噬菌体，我们分离出了相应的T2和T6克隆。对三个αgt基因的核苷酸序列以及推导得到的氨基酸序列进行了比较。从结构、功能和进化方面对这些基因及其产物之间的差异进行了讨论。

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Nature. 1956 Sep 22;178(4534):637. doi: 10.1038/178637a0.
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A new pyrimidine base from bacteriophage nucleic acids.一种来自噬菌体核酸的新型嘧啶碱基。
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A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
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New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
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Alignment of a restriction map with the genetic map of bacteriophage T4.限制性酶切图谱与噬菌体T4遗传图谱的比对。
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