Zheng Huangtao, Lin Shixian, Chen Peng R
Peking-Tsinghua Center for Life Sciences, Beijing, China.
Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
Methods Mol Biol. 2018;1728:113-126. doi: 10.1007/978-1-4939-7574-7_6.
The genetic code expansion strategy has become an elegant method for site-specific incorporation of noncanonical amino acids with diverse functionalities into proteins of interest in bacteria, yeast, mammalian cells, and even animals. This technique allows precise labeling as well as manipulation of a given protein to dissect its physiological and/or pathological roles under living conditions. Here, we demonstrate the extension of a recently emerged pyrrolysine-based genetic code expansion strategy for encoding noncanonical amino acids into enteric bacterial pathogens.
遗传密码扩展策略已成为一种巧妙的方法，可将具有多种功能的非天然氨基酸位点特异性地掺入细菌、酵母、哺乳动物细胞甚至动物体内的目标蛋白质中。这项技术能够实现精确标记以及对特定蛋白质的操控，从而在活体条件下剖析其生理和/或病理作用。在此，我们展示了一种最近出现的基于吡咯赖氨酸的遗传密码扩展策略在肠道细菌病原体中编码非天然氨基酸的拓展应用。
