巴西流行地区鼻腔样本中分枝杆菌结核分歧杆菌分离株的 VNTR 分析基因分型比较。
Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region.
机构信息
a Faculdade de Medicina, Departamento de Patologia e Medicina Legal , Federal University of Ceará , Fortaleza , Brazil.
b Seção de Bacteriologia e Micologia , Instituto Evandro Chagas , Ananindeua , Brazil.
出版信息
Pathog Glob Health. 2018 Mar;112(2):79-85. doi: 10.1080/20477724.2018.1427308. Epub 2018 Feb 6.
This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Ceará. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS.
本研究通过 MIRU-VNTR 分析了鼻腔分枝杆菌分离株的遗传多样性，并与流行病学和临床数据相关。该样本由 48 例新诊断的麻风病病例组成，这些病例的鼻腔分泌物 (NS) 中检测到麻风分枝杆菌 PCR 阳性，他们均在巴西福塔雷萨的国家皮肤病学参考中心 (CDERM) 就诊。从每个患者的 NS 中提取总 DNA，并用于扩增四个麻风分枝杆菌 VNTR 基因座。形成了四个具有相同基因型的麻风分枝杆菌分离株簇。在空间分析中，12 例麻风病患者呈现出相似的基因型，组织成 4 个簇。在本研究中最常见的基因型是 AC8b:8、AC9:7、AC8a:8、GTA9:10，这可能代表在塞阿拉州流行的菌株基因型。证明了最小的一组四个 MIRU-VNTR 基因座可用于研究 NS 中麻风分枝杆菌分离株的遗传多样性。
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