Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska 68178, USA.
Chongqing Academy of Animal Science, Chongqing 402460, China.
Sci Data. 2018 Feb 6;5:180005. doi: 10.1038/sdata.2018.5.
Although hair cells are the sensory receptors of the auditory and vestibular systems in the ears of all vertebrates, hair cell properties are different between non-mammalian vertebrates and mammals. To understand the basic biological properties of hair cells from non-mammalian vertebrates, we examined the transcriptome of adult zebrafish auditory and vestibular hair cells. GFP-labeled hair cells were isolated from inner-ear sensory epithelia of a pou4f3 promoter-driven GAP-GFP line of transgenic zebrafish. One thousand hair cells and 1,000 non-sensory surrounding cells (nsSCs) were separately collected for each biological replicate, using the suction pipette technique. RNA sequencing of three biological replicates for the two cell types was performed and analyzed. Comparisons between hair cells and nsSCs allow identification of enriched genes in hair cells, which may underlie hair cell specialization. Our dataset provides an extensive resource for understanding the molecular mechanisms underlying morphology, function, and pathology of adult zebrafish hair cells. It also establishes a framework for future characterization of genes expressed in hair cells and the study of hair cell evolution.
虽然毛细胞是所有脊椎动物耳朵中听觉和前庭系统的感觉受体，但在非哺乳动物和哺乳动物之间，毛细胞的特性是不同的。为了了解非哺乳动物脊椎动物毛细胞的基本生物学特性，我们研究了成年斑马鱼听觉和前庭毛细胞的转录组。使用 GFP 标记的毛细胞从 pou4f3 启动子驱动的 GAP-GFP 线的转基因斑马鱼内耳感觉上皮中分离出来。使用吸管技术，每个生物学重复分别收集了 1000 个毛细胞和 1000 个非感觉周围细胞（nsSCs）。对两种细胞类型的三个生物学重复进行了 RNA 测序和分析。毛细胞和 nsSCs 之间的比较可以确定毛细胞中富集的基因，这些基因可能是毛细胞特化的基础。我们的数据集为理解成年斑马鱼毛细胞的形态、功能和病理学的分子机制提供了广泛的资源。它还为未来研究毛细胞中表达的基因和毛细胞进化奠定了基础。
