School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
Department of Antibiotics, Heilongjiang Institute for Food and Drug Control , Harbin 150080, People's Republic of China.
J Nat Prod. 2018 Feb 23;81(2):335-342. doi: 10.1021/acs.jnatprod.7b00782. Epub 2018 Feb 6.
The medicinal plant Catharanthus roseus accumulates large numbers of terpenoid indole alkaloids (TIAs), including the pharmaceutically important vinblastine, vincristine, ajmalicine, and serpentine. The phytohormone ethylene or methyl jasmonate (MeJA) can markedly enhance alkaloid accumulation. The interaction between ethylene or MeJA in the regulation of TIA biosynthesis in C. roseus is unknown. Here, a metabolomics platform is reported that is based on liquid chromatography (LC) coupled with time-of-flight mass spectrometry to study candidate components for TIA biosynthesis, which is controlled by ethylene or MeJA in C. roseus. Multivariate analysis identified 16 potential metabolites mostly associated with TIA metabolic pathways and seven targeted metabolites, outlining the TIA biosynthesis metabolic networks controlled by ethylene or MeJA. Interestingly, ethylene and MeJA regulate the 2-C-methyl-d-erythritol 4-phosphate (MEP) and acetate-mevalonate (MVA) pathways through AACT and HMGS and through DXS, respectively, to induce TIA biosynthesis in C. roseus. Overall, both nontargeted and targeted metabolomics, as well as transcript analysis, were used to reveal that MeJA and ethylene control different metabolic networks to induce TIA biosynthesis.
药用植物长春花积累了大量的萜吲哚生物碱（TIAs），包括具有重要药用价值的长春碱、长春新碱、阿马碱和蛇根碱。植物激素乙烯或茉莉酸甲酯（MeJA）可以显著促进生物碱的积累。乙烯或 MeJA 在长春花 TIAs 生物合成调节中的相互作用尚不清楚。本研究报道了一种基于液相色谱（LC）与飞行时间质谱联用的代谢组学平台，用于研究由乙烯或 MeJA 控制的长春花 TIAs 生物合成的候选成分。多变量分析确定了 16 个与 TIAs 代谢途径相关的潜在代谢物和 7 个靶向代谢物，概述了由乙烯或 MeJA 控制的 TIAs 生物合成代谢网络。有趣的是，乙烯和 MeJA 通过 AACT 和 HMGS 以及分别通过 DXS 调节 2-C-甲基-D-赤藓醇 4-磷酸（MEP）和乙酸甲羟戊酸（MVA）途径，以诱导长春花中的 TIAs 生物合成。总之，非靶向和靶向代谢组学以及转录分析都揭示了 MeJA 和乙烯控制不同的代谢网络来诱导 TIAs 生物合成。
