Department of Dental Materials, Peking University School and Hospital of Stomatology, China; National Engineering Laboratory for Digital and Material Technology of Stomatology, China; Beijing Key Laboratory of Digital Stomatology, China.
Department of Dental Materials, Peking University School and Hospital of Stomatology, China; National Engineering Laboratory for Digital and Material Technology of Stomatology, China; Beijing Key Laboratory of Digital Stomatology, China.
Mater Sci Eng C Mater Biol Appl. 2018 Apr 1;85:182-190. doi: 10.1016/j.msec.2017.12.032. Epub 2017 Dec 29.
The primary amine along the chitosan backbone plays a key role in biomedical applications. Although chitosan-based porous scaffolds have been widely used in tissue engineering, it remains very challenging to regulate uncross-linking primary amine content (C) in scaffolds in order to realize particular mechanical and biological properties. In the present study, chitosan/tripolyphosphate (TPP) scaffolds with controlled C (i.e., degree of cross-linking) were prepared based on the ionic-dependent solubility of chitosan together with the freezing process. The effects of the concentration of TPP (C) and NaCl (C) in the cross-linking solution on C were studied by infrared spectroscopy, ninhydrin assays and elemental analysis. The results showed that C decreased with increasing C and decreasing C. C affected physicomechanical properties such as swelling behavior and the mechanical strength of the chitosan/TPP scaffolds. The uncross-linking primary amine in scaffolds can be used for chemical and biological modifications. The protein loading of the scaffolds demonstrated that the pH-responsive adsorption and release behavior was influenced by C. Cell experiments also illustrated that C affected the proliferation of bone marrow mesenchymal stem cells (BM-MSCs). All of these results indicate that these porous chitosan/TPP scaffolds containing uncross-linking primary amines are potentially useful for applications in regenerative bone medicine.
壳聚糖骨架上的伯胺在生物医学应用中起着关键作用。尽管基于壳聚糖的多孔支架已广泛应用于组织工程，但调控支架中未交联伯胺含量（C）以实现特定的机械和生物学性能仍然极具挑战性。在本研究中，基于壳聚糖的离子依赖性溶解度以及冷冻过程，制备了具有可控 C（即交联度）的壳聚糖/三聚磷酸钠（TPP）支架。通过红外光谱、茚三酮分析和元素分析研究了 TPP（C）和 NaCl（C）在交联溶液中的浓度对 C 的影响。结果表明，C 随 C 的增加和 C 的降低而降低。C 影响了壳聚糖/TPP 支架的物理机械性能，如溶胀行为和力学强度。支架中的未交联伯胺可用于化学和生物学修饰。支架的蛋白质负载表明，C 影响 pH 响应性吸附和释放行为。细胞实验也表明，C 影响骨髓间充质干细胞（BM-MSCs）的增殖。所有这些结果表明，这些含有未交联伯胺的多孔壳聚糖/TPP 支架在再生骨医学应用中具有潜在的用途。
