Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Diagonal 113 y 61, La Plata, Buenos Aires, 1900, Argentina; Facultad de Ciencias Agrarias y Forestales, UNLP, La Plata, Argentina.
Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Diagonal 113 y 61, La Plata, Buenos Aires, 1900, Argentina; Physical Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires-CONICET, Junín 956, Buenos Aires, C1113AAD, Argentina.
J Plant Physiol. 2018 Mar;222:51-58. doi: 10.1016/j.jplph.2018.01.001. Epub 2018 Jan 4.
Improving phosphorus (P) acquisition and utilization in crops is of great importance in order to achieve a good plant nutritional state and maximize biomass production while minimizing the addition of fertilizers, and the concomitant risk of eutrophication. This study explores to which extent key processes involved in P-acquisition, and other acclimation mechanisms to low P supply in maize (Zea mays L.) plants, are affected by the addition of a nitric oxide (NO) donor (S-nitrosoglutathione, GSNO). Plants grown in a complete culture solution were exposed to four treatments performed by the combination of two P levels (0 and 0.5 mM), and two GSNO levels (0 and 0.1 mM), and responses to P-deprivation were then studied. Major plant responses related to P-deprivation were affected by the presence of the NO donor. In roots, the activity of acid phosphatases was significantly increased in P-depleted plants simultaneously exposed to GSNO. Acidification of the culture solution also increased in plants that had been grown in the presence of the NO donor. Furthermore, the potential capability displayed by roots of P-deprived plants for P-uptake, was higher in the plants that had been treated with GSNO. These results indicate that exogenous NO addition affects fundamental acclimation responses of maize plants to P scarcity, particularly and positively those that help plants to sustain P-acquisition under low P availability.
为了实现良好的植物营养状态，最大限度地提高生物量生产，同时最大限度地减少肥料的添加和富营养化的风险，提高作物对磷（P）的获取和利用非常重要。本研究探讨了一氧化氮（NO）供体（GSNO）的添加在多大程度上影响了玉米（Zea mays L.）植株中磷获取的关键过程和其他适应低磷供应的机制。在完全培养液中生长的植物暴露于四种处理方法中，这些处理方法是通过两种磷水平（0 和 0.5 mM）和两种 GSNO 水平（0 和 0.1 mM）的组合进行的，然后研究了对磷饥饿的响应。与磷饥饿相关的主要植物响应受 NO 供体的存在影响。在根系中，同时暴露于 GSNO 的磷耗尽植物中酸性磷酸酶的活性显著增加。在存在 NO 供体的情况下生长的植物中，培养液的酸化也增加了。此外，在 GSNO 处理的植物中，磷饥饿植物根系的磷吸收能力更高。这些结果表明，外源 NO 添加会影响玉米植株对磷匮乏的基本适应响应，特别是有助于植物在低磷可用性下维持磷获取的那些响应。
