Haematex Research Pty Ltd, Sydney, Australia.
Haematology Department, PathWest, Queen Elizabeth II Medical Centre, Perth, Australia.
Thromb Res. 2018 Mar;163:117-122. doi: 10.1016/j.thromres.2018.01.047. Epub 2018 Jan 31.
To evaluate a simple method using an adsorbent product (DOAC Stop) for extracting direct oral anti-coagulants (DOACs) from plasmas.
DOAC Stop was tested on normal and a range of abnormal plasmas initially using activated partial thromboplastin time (APTT) tests and a more DOAC-sensitive Russells viper venom-based clotting test (DOAC Test). Further tests for prothrombin time/International Normalized Ratio (PT/INR), lupus anticoagulants, activated protein C (APC) resistance, antithrombin, plasminogen, protein C and S were carried out on various patient samples.
DOAC Stop was found to remove all types of DOACs including dabigatran, apixaban, rivaroxaban and edoxaban from test plasmas with minimal effect on any of the (mainly clotting) tests considered in this study.
DOAC Stop can be used to identify plasmas containing DOAcs using simple clotting tests. It reduces the false positivity for lupus anticoagulants observed in dilute Russells viper venom time (dRVVT) tests on DOAC-containing plasmas and could be useful for eliminating unwanted effects of DOACs on routine coagulation testing.
评估一种使用吸附剂产品（DOAC Stop）从血浆中提取直接口服抗凝剂（DOACs）的简单方法。
首先使用活化部分凝血活酶时间（APTT）试验和更敏感的基于响尾蛇蛇毒的凝血试验（DOAC Test）对 DOAC Stop 进行了正常和一系列异常血浆的测试。进一步对各种患者样本进行凝血酶原时间/国际标准化比值（PT/INR）、狼疮抗凝剂、活化蛋白 C（APC）抵抗、抗凝血酶、纤溶酶原、蛋白 C 和 S 的检测。
DOAC Stop 被发现可以从测试血浆中去除所有类型的 DOACs，包括达比加群、阿哌沙班、利伐沙班和依度沙班，对本研究中考虑的任何（主要是凝血）试验几乎没有影响。
DOAC Stop 可用于使用简单的凝血试验识别含有 DOAcs 的血浆。它减少了在含有 DOAC 的血浆中进行稀释响尾蛇蛇毒时间（dRVVT）试验时观察到的狼疮抗凝剂的假阳性，并可能有助于消除 DOAC 对常规凝血检测的不必要影响。
