INRA, UR370 Qualité des Produits Animaux, F-63122 Saint Genès Champanelle, France.
INRA, UR SQPOV, F-84000 Avignon, France.
Food Chem. 2018 May 30;249:111-118. doi: 10.1016/j.foodchem.2018.01.013. Epub 2018 Jan 2.
We studied the kinetics of peptide release during the gastric digestion of meat proteins in vivo, in view to predicting the release of bioactive peptides further on in the digestive tract. Six mini pigs fitted with gastric cannulas received a meal with cooked beef as protein source. Digesta was collected at regular time intervals up to 5½ h. The peptides generated by the gastric digestion of meat were identified and quantified using label-free LC MS, thereafter subjected to in silico digestion mimicking the action of intestinal enzymes. Three clusters of proteins presenting similar evolutions according to their dynamic hydrolysis were obtained. This study clearly improves the in silico prediction of the intestinal release of bioactive peptides by mapping meat protein degradation in the stomach in an in vivo model. Knowledge of the conformation of the peptides released in the stomach further improves this prediction.
我们研究了体内肉蛋白胃消化过程中肽释放的动力学，以期进一步预测肽在消化道中的释放。六头装有胃管的小型猪接受了以煮牛肉为蛋白质来源的餐食。在 5 个半小时内，定期收集消化物。使用无标记 LC-MS 鉴定和定量胃消化生成的肽，然后对其进行模拟肠道酶作用的计算机消化。根据其动态水解，得到了三个具有相似演变的蛋白质簇。这项研究通过在体内模型中映射胃中的肉蛋白降解，明显提高了生物活性肽肠内释放的计算机预测。对胃中释放的肽构象的了解进一步提高了这种预测。
