Chembio Diagnostic Systems, Inc., 3661 Horseblock Road, Medford, NY 11763, USA.
SaBio Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13005 Ciudad Real, Spain.
Vet Microbiol. 2018 Feb;214:89-92. doi: 10.1016/j.vetmic.2017.12.013. Epub 2017 Dec 20.
Using multi-antigen print immunoassay and DPP VetTB Assay approved in the United States for testing captive cervids and elephants, we analyzed antibody recognition of MPB83 and CFP10/ESAT-6 antigens in Asian elephants (Elephas maximus) infected with Mycobacterium tuberculosis and in white-tailed deer (Odocoileus virginianus), fallow deer (Dama dama), elk (Cervus elaphus), and cattle (Bos taurus) infected with Mycobacterium bovis. Serum IgG reactivity to MPB83 was found in the vast majority of tuberculous cattle and cervid species among which white-tailed deer and elk also showed significant CFP10/ESAT-6 recognition rates with added serodiagnostic value. In contrast, the infected elephants developed antibody responses mainly to CFP10/ESAT-6 with MPB83 reactivity being relatively low. The findings demonstrate distinct patterns of predominant antigen recognition by different animal hosts in tuberculosis.
我们使用了美国批准的多抗原打印免疫分析和 DPP VetTB 检测试剂盒，对感染结核分枝杆菌的亚洲象（Elephas maximus）和感染牛分枝杆菌的白尾鹿（Odocoileus virginianus）、黇鹿（Dama dama）、麋鹿（Cervus elaphus）和牛（Bos taurus）进行了 MPB83 和 CFP10/ESAT-6 抗原的抗体识别分析。在大多数感染结核分枝杆菌的牛和鹿种中，我们发现了针对 MPB83 的 IgG 反应，其中白尾鹿和麋鹿对 CFP10/ESAT-6 的识别率也很高，具有额外的血清学诊断价值。相比之下，感染的大象主要针对 CFP10/ESAT-6 产生抗体反应，而对 MPB83 的反应相对较低。这些发现表明，不同的动物宿主在结核病中存在明显的主要抗原识别模式。
