Utilization of centrate from urban wastewater plants for the production of Scenedesmus sp. in a raceway-simulating reactor.

This work investigates the production of the native microalgae strain Scenedesmus sp. in semi-continuous mode at lab scale in open raceway-simulating reactors and using centrate as the culture medium. The biomass productivity and nutrient removal capacity of Scenedesmus sp. at different dilution rates were investigated indoors as well as its tolerance to centrate as the culture medium at different concentrations. A biomass productivity of 7.80 g/m day was obtained at 200 μE/m s, 5 cm culture depth, 0.30 1/day of dilution rate and 60% centrate while nitrogen and phosphorus removal rates were 1.50 g/m day and 0.15 g/m day, respectively. The produced biomass characterization under these conditions showed a lipid content of 12.60% d wt. along with a favorable fatty acids profile with 57.70% of total fatty acids composed of saturated and monounsaturated fatty acids. Subsequently, the effect of light intensity and culture depth on biomass productivity and nutrient uptake as well as the biochemical composition and fatty acids profile was studied using two irradiance levels (200 and 1000 μE/m s) and four culture depths (5 cm, 10 cm, 15 cm and 20 cm). Under optimal conditions of 1000 μE/m s, 60% centrate, 0.30 1/day dilution rate and 15 cm culture depth, a maximum biomass productivity of 22.20 g/m day was obtained. Nitrogen and phosphorus removal rates of 2.00 gN/m day and 0.40 gP/m day, respectively, were recorded. An amount of 11.70% d wt. of lipids was determined along with a suitable fatty acids profile for biofuel production.