Algae R & D Centre, Environmental and Conservation Sciences, College of Science, Health, Engineering and Education School, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia; Centre of Water, Energy, Waste, Harry Butler Institute, Murdoch University, Murdoch, WA 6150, Australia.
Algae R & D Centre, Environmental and Conservation Sciences, College of Science, Health, Engineering and Education School, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
Sci Total Environ. 2021 Jun 25;775:145853. doi: 10.1016/j.scitotenv.2021.145853. Epub 2021 Feb 14.
The successful cultivation of microalgae in wastewater establishes a waste to profit scenario as it combines treatment of a waste stream with production of valuable end-products. Here, growth and nutrient removal efficiency of three different locally isolated microalgal cultures (Chlorella sp., Scenedesmus sp., and a mixed consortium) cultivated in anaerobically digested municipal centrate (ADMC) and anaerobically digested abattoir effluent (ADAE) was evaluated. No significant differences (P > 0.05) in specific growth rate and biomass productivity were recorded between Chlorella monocultures and the mixed culture grown in both effluents. Scenedesmus sp. monocultures was found incapable of growth in both ADMC and ADAE throughout the cultivation period resulting in the collapse of cultures and no further measurements on the growth, biomass production and nutrient removal efficiency of this alga in both effluent. F´/F´ values which represent the immediate photo-physiological status of microalgae found to be negatively inhibited when Scenedesmus sp. was grown in both effluents throughout the cultivation period. F´/F´ values of Chlorella sp. monocultures and the mixed cultures recovered back to normal (≈0.6) after an initial drop. Ammonium removal rates was found to be significantly higher (≈2 folds) for Chlorella sp. monocultures grown in both ADMC and ADAE when compared to the mixed cultures. Nonetheless, no significant differences were observed in the removal of phosphate for both cultures in the different effluents. The total protein and carbohydrate content of the biomass produced was similar for both microalgae cultures grown using ADAE and ADMC. However, chlorophyll a and total carotenoids content were found to be higher (P < 0.05) for the cultures grown in ADAE than ADMC. Overall, Chlorella sp. monoculture was the most efficient option for treating both ADMC and ADAE while simultaneously generating protein rich biomass (up to 49%) that can be potentially exploited as aquaculture feedstock.
在废水成功培养微藻建立了一种废物变利润的情况，因为它将废水处理与有价值的最终产品生产结合在一起。在这里，评价了在厌氧消化的城市浓缩物（ADMC）和厌氧消化的屠宰废水（ADAE）中培养的三种不同的本地分离的微藻培养物（绿藻，栅藻和混合生物量）的生长和养分去除效率。在两种废水中，绿藻纯培养物和混合培养物的比生长速率和生物量生产力没有显着差异（P> 0.05）。在整个培养过程中，栅藻纯培养物无法在 ADMC 和 ADAE 中生长，导致培养物崩溃，并且不再对该藻类在两种废水中的生长，生物量生产和养分去除效率进行进一步测量。F´/F´值代表微藻的即时光生理状态，发现当 Scenedesmus sp.在整个培养过程中在两种废水中生长时，其受到负抑制。绿藻纯培养物和混合培养物的 F´/F´值在初始下降后恢复正常（≈0.6）。与混合培养物相比，在 ADMC 和 ADAE 中生长的绿藻纯培养物的氨去除率明显更高（≈2 倍）。尽管如此，在不同的废水中，两种培养物的磷酸盐去除率没有明显差异。使用 ADAE 和 ADMC 培养的两种微藻的生物量的总蛋白和碳水化合物含量相似。然而，与 ADMC 相比，在 ADAE 中生长的培养物的叶绿素 a和总类胡萝卜素含量更高（P <0.05）。总体而言，绿藻纯培养物是处理 ADMC 和 ADAE 同时产生富含蛋白质的生物量（高达 49％）的最有效选择，该生物量可潜在地用作水产养殖饲料。
