Vitale M L, de las Nieves Parisi M, Chiocchio S R, Tramezzani J H
Acta Physiol Pharmacol Latinoam. 1985;35(4):473-9.
The study examined the effect of serotonin (5-HT) on gonadotrophin release and its tissue site of action using a sequential double chamber perifusion system. Cycling female rats were killed between 12.00 and 13.00 h on the day of proestrus. Median eminences and anterior pituitaries were removed and transferred to perifusion chambers. Two types of experiments were performed: a) five median eminences (ME) were placed in the first chamber and one anterior pituitary in the second chamber. b) In the second group, only the anterior pituitary was perifused. The effluent from the first chamber perifused the second chamber. The effluent from the second chamber was collected for hormone assays. Addition of serotonin (final concentration 0.06, 0.6 and 6.0 uM) stimulated the release of LH and FSH into the perifusion fluid draining the pituitary in series with the median eminences. Pretreatment with the 5-HT receptor blocker cyproheptadine (1 uM) completely inhibited the stimulatory effect of 5-HT. Serotonin was ineffective in stimulating gonadotrophin release when injected into the tube connecting the first and the second chambers (50 ul of 0.6 uM solution) or when the anterior pituitary was perifused alone. Serotonin did not affect the prolactin release in any of the experimental conditions studied. These observations demonstrate that serotonin stimulated the gonadotrophin release by acting on serotoninergic receptors at the level of the median eminence.
本研究使用连续双室灌流系统，研究了血清素（5-羟色胺，5-HT）对促性腺激素释放的影响及其作用的组织部位。在动情前期当天的12:00至13:00之间处死处于发情周期的雌性大鼠。取出正中隆起和垂体前叶并转移至灌流室。进行了两种类型的实验：a）将五个正中隆起（ME）置于第一室，一个垂体前叶置于第二室。b）在第二组中，仅对垂体前叶进行灌流。来自第一室的流出液灌流第二室。收集来自第二室的流出液进行激素测定。添加血清素（终浓度为0.06、0.6和6.0 μM）可刺激促黄体生成素（LH）和促卵泡生成素（FSH）释放到与正中隆起串联的垂体灌流液中。用5-HT受体阻滞剂赛庚啶（1 μM）预处理可完全抑制5-HT的刺激作用。当将血清素注入连接第一室和第二室的管中（50 μl的0.6 μM溶液）或单独灌流垂体前叶时，血清素对刺激促性腺激素释放无效。在所研究的任何实验条件下，血清素均不影响催乳素的释放。这些观察结果表明，血清素通过作用于正中隆起水平的5-羟色胺能受体来刺激促性腺激素释放。
