Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Int J Infect Dis. 2018 Apr;69:26-28. doi: 10.1016/j.ijid.2018.01.024. Epub 2018 Feb 1.
To date, at least 50 species of Legionella have been described. These organisms are ubiquitous in nature and have been isolated from diverse ecological environments, including man-made structures such as cooling towers and spas. Legionellae have also been isolated from human and veterinary clinical specimens, and their roles in disease are well-established. This report describes the isolation of a novel Legionella species from a respiratory specimen from a patient with influenza and suspected pulmonary embolus.
A 68-year-old male presented to an Indianapolis-area hospital with pulmonary disease; upon workup, he was found to have influenza A. Bronchoalveolar lavage fluid was also submitted for conventional bacterial culture and Legionella culture. The patient was prescribed a broad-spectrum antibiotic and recovered.
A Legionella-like bacterium was isolated on buffered charcoal yeast extract agar, and mass spectrometry and comparative 16S rRNA gene sequencing inconclusively identified the isolate as a Legionella sp. Further analysis of the 16S rRNA gene confirmed the strain to be a new species, related to Legionella hackeliae. Physiochemical and morphological testing were used to confirm the discovery of a novel species, Legionella indianapolisensis sp. nov., type strain SMNF-IS.
迄今为止，至少有 50 种军团菌已被描述。这些生物在自然界中无处不在，已从包括冷却塔和温泉在内的各种生态环境中分离出来。军团菌也已从人类和兽医临床标本中分离出来，其在疾病中的作用已得到充分证实。本报告描述了从一名患有流感和疑似肺栓塞的患者的呼吸道标本中分离出一种新型军团菌的情况。
一名 68 岁男性因肺部疾病就诊于印第安纳波利斯地区医院；在进行检查时，发现他患有甲型流感。还提交了支气管肺泡灌洗液进行常规细菌培养和军团菌培养。患者被开了一种广谱抗生素，随后康复。
在缓冲炭酵母提取物琼脂上分离出一种类似军团菌的细菌，质谱和比较 16S rRNA 基因测序不确定地将分离株鉴定为军团菌属。对 16S rRNA 基因的进一步分析证实该菌株为一种新物种，与军团菌 hackeliae 有关。生理化学和形态学测试用于确认一种新型物种的发现，即新型军团菌印第安纳波利斯亚种，其模式株为 SMNF-IS。
