Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
Int J Biol Macromol. 2018 Jun;112:745-753. doi: 10.1016/j.ijbiomac.2018.01.191. Epub 2018 Feb 1.
Organoselemium compounds possess strong antioxidant activity as well as protecting cells from DNA damage, mitochondrial injury, lipid peroxidation, protein denaturation and cell death. Herein, we used an in vitro oxidative model to further investigate the antioxidant effects of a novel organoselemium compound, low molecular-weight seleno-aminopolysaccharides (LSA) in intestinal porcine epithelial cells (IPEC-1), and the molecular mechanisms of these effects. Analysis by MTT assay showed that LSA could significantly increase the viability of IPEC-1 cells compared to cells exposed to HO. We found that the levels of different antioxidant enzymes could dramatically increase in LSA pretreatment group compared to HO treatment group. Furthermore, LSA significantly increased the gene expression of antioxidant enzymes and phase 2 detoxifying enzymes in IPEC-1 cells, as measured by qRT-PCR. In addition, LSA up-regulated the expression level of intracellular transcription factor NF-E2-related factor 2 (Nrf2) and inhibited the level of kelch-like ECH-associated protein 1 (Keap1) with western blot analysis. Collectively, the present study suggested that LSA has the protective effect of IPEC-1 cells against HO-induecd oxidative stress, and its mechanism may be related to activation of Keap1/Nrf2 signaling pathway in intestinal epithelial cells.
有机硒化合物具有很强的抗氧化活性，能保护细胞免受 DNA 损伤、线粒体损伤、脂质过氧化、蛋白质变性和细胞死亡。在此，我们使用体外氧化模型进一步研究了一种新型有机硒化合物、低分子硒-氨基多糖（LSA）在猪肠上皮细胞（IPEC-1）中的抗氧化作用及其作用机制。MTT 分析表明，与接触 HO 的细胞相比，LSA 可显著提高 IPEC-1 细胞的活力。我们发现，与 HO 处理组相比，LSA 预处理组中不同抗氧化酶的水平可显著增加。此外，LSA 通过 qRT-PCR 显著增加了 IPEC-1 细胞中抗氧化酶和相 2 解毒酶的基因表达。此外，LSA 通过 Western blot 分析上调了细胞内转录因子 NF-E2 相关因子 2（Nrf2）的表达水平，并抑制了 kelch 样 ECH 相关蛋白 1（Keap1）的水平。综上所述，本研究表明 LSA 对 IPEC-1 细胞具有抵抗 HO 诱导的氧化应激的保护作用，其机制可能与肠上皮细胞中 Keap1/Nrf2 信号通路的激活有关。
