Sugihara Asami, Nguyen Luan Cao, Shamim Hossain Mohammad, Iida Tetsushi, Nakase Mai, Takegawa Kaoru, Senda Mitsuhisa, Jida Shohei, Ueno Masaru
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.
Division of Cytogenetics, National Institute of Genetics, Mishima, Shizuoka, Japan; Department of Genetics, School of Life Science, Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Shizuoka, 411-8540, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 4-1-8, Honcho, Kawaguchi, Saitama, 332-0012, Japan.
Biochem Biophys Res Commun. 2018 Feb 19;496(4):1284-1290. doi: 10.1016/j.bbrc.2018.02.001. Epub 2018 Feb 2.
Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1 causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1Δ. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1Δ. We found phosphoinositol 4-phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1Δ pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1 is required for the maintenance of circular chromosomes.
