Zhu Huan, Qiao Lixing, Sun Yao, Yin Liping, Huang Li, Jiang Li, Li Jiaqing
Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, Jiangsu Province 210009, China.
Rehabilitation Department, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu Province 210008, China.
Neurosci Lett. 2018 Apr 23;673:67-72. doi: 10.1016/j.neulet.2018.01.046. Epub 2018 Mar 20.
Perinatal hypoxic-ischemic insult is considered a major contributor to child mortality and morbidity and leads to neurological deficits in newborn infants. There has been a lack of promising neurotherapeutic interventions for hypoxic-ischemic brain damage (HIBD) for clinical application in infants. The present study aimed to investigate the correlation between neurogenesis and basic fibroblast growth factor (bFGF) in the hippocampal dentate gyrus (DG) region in neonatal rats following HIBD.
Cell proliferation was examined by detecting BrdU signals, and the role of bFGF in cell proliferation in the DG region following neonatal HIBD was investigated.
Cell proliferation was induced by HIBD in the hippocampal DG of neonatal rats. Furthermore, bFGF gene expression was upregulated in the hippocampus in neonatal rats, particularly between 7 and 14 days after HIBD. Moreover, intraperitoneal injection of exogenous bFGF enhanced cell proliferation in the hippocampal DG following neonatal HIBD.
Taken together, these data indicate that cell proliferation in the DG could be induced by neonatal HIBD, and bFGF promotes proliferation following neonatal HIBD.
围产期缺氧缺血性损伤被认为是导致儿童死亡和发病的主要因素，并会导致新生儿出现神经功能缺损。目前缺乏有前景的针对缺氧缺血性脑损伤（HIBD）的神经治疗干预措施用于婴儿的临床应用。本研究旨在探讨新生大鼠HIBD后海马齿状回（DG）区域神经发生与碱性成纤维细胞生长因子（bFGF）之间的相关性。
通过检测BrdU信号来检查细胞增殖情况，并研究bFGF在新生大鼠HIBD后DG区域细胞增殖中的作用。
新生大鼠海马DG区的HIBD可诱导细胞增殖。此外，新生大鼠海马中bFGF基因表达上调，尤其是在HIBD后7至14天之间。而且，腹腔注射外源性bFGF可增强新生大鼠HIBD后海马DG区的细胞增殖。
综上所述，这些数据表明新生大鼠HIBD可诱导DG区细胞增殖，且bFGF可促进新生大鼠HIBD后的增殖。
