Department of Hepatobiliary Surgery, Peking University Shenzhen Hospital, Shenzhen, China.
Center Laboratory, Peking University Shenzhen Hospital, Shenzhen, China.
Biomed Res Int. 2017;2017:4107943. doi: 10.1155/2017/4107943. Epub 2017 Dec 19.
Transplantation of islets is a promising alternative to treat type 1 diabetes (T1D), but graft rejection is the major obstacle to its application in clinical practice. We evaluated the effects of mesenchymal stem cells (MSCs) and immature dendritic cells (imDCs) on islet transplantation in diabetic model.
The streptozotocin T1D model was established in BABL/c mice. Rat islets were isolated and identified with dithizone (DTZ) staining. MSCs and imDCs were isolated from bone marrow of syngenic mice. Islets, alone or along with MSCs and/or imDCs, were transplanted to the left kidney capsule of diabetic mice. The blood glucose levels and glycosylated hemoglobin levels after transplantation were monitored.
Cotransplantation significantly decreased blood glucose and glycosylated hemoglobin levels in the diabetes mice. Transplantation of 200 islets + 2 × 10 MSCs + 2 × 10 imDCs could not only restore normal blood glucose levels, but also significantly prolong graft survival for 12.6 ± 3.48 days.
Cotransplantation of allogenic islets with imDCs and/or MSCs can significantly promote graft survival, reverse hyperglycemia, and effectively control the glycosylated hemoglobin levels.
胰岛移植是治疗 1 型糖尿病（T1D）的一种很有前途的方法，但移植物排斥仍是其在临床应用中的主要障碍。我们评估了间充质干细胞（MSCs）和未成熟树突状细胞（imDCs）对胰岛移植治疗糖尿病模型的影响。
链脲佐菌素诱导 BABL/c 小鼠 T1D 模型，用二硫代丁二酸钠（DTZ）染色鉴定分离的胰岛。从同基因小鼠的骨髓中分离 MSC 和 imDC。将胰岛，单独或与 MSCs 和/或 imDC 一起，移植到糖尿病小鼠的左肾包膜中。监测移植后的血糖和糖化血红蛋白水平。
共移植可显著降低糖尿病小鼠的血糖和糖化血红蛋白水平。移植 200 个胰岛+2×10 MSC+2×10 imDC 不仅可以恢复正常的血糖水平，还可以显著延长移植物存活时间至 12.6±3.48 天。
同种异体胰岛与 imDC 和/或 MSC 共移植可以显著促进移植物存活，逆转高血糖，并有效控制糖化血红蛋白水平。
