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zymosan-A 对小鼠的放射防护作用机制。

The mechanism for the radioprotective effects of zymosan-A in mice.

机构信息

Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.

出版信息

J Cell Mol Med. 2018 Apr;22(4):2413-2421. doi: 10.1111/jcmm.13538. Epub 2018 Feb 7.

DOI:10.1111/jcmm.13538
PMID:29411511
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5867165/
Abstract

It proved that Zymosan-A protected the haematopoietic system from radiation-induced damage via Toll-Like Receptor2 in our previous study. In this study, we investigated the potential mechanism for the radioprotective effects of Zymosan-A. The mice were treated with Zymosan-A (50 mg/kg, dissolved in NS) via peritoneal injection 24 and 2 hours before ionizing radiation. Apoptosis of bone marrow cells and the levels of IL-6, IL-12, G-CSF and GM-CSF were evaluated by flow cytometry assay. DNA damage was determined by γ-H2AX foci assay. In addition, RNA sequencing was performed to identify differentially expressed genes (DEGs). Zymosan-A protected bone marrow cells from radiation-induced apoptosis, up-regulated IL-6, IL-12, G-CSF and GM-CSF in bone marrow cells. Zymosan-A also protected cells from radiation-induced DNA damage. Moreover, RNA sequencing analysis revealed that Zymosan-A induced 131 DEGs involved in the regulation of immune system process and inflammatory response. The DEGs were mainly clustered in 18 KEGG pathways which were also associated with immune system processes. Zymosan-A protected bone marrow cells from radiation-induced apoptosis and up-regulated IL-6, IL-12, G-CSF and GM-CSF. Moreover, Zymosan-A might also exhibit radioprotective effects through regulating immune system process and inflammatory response. These results provided new knowledge regarding the radioprotective effect of Zymosan-A.

摘要

在我们之前的研究中已证实，酵母聚糖 A 通过 Toll 样受体 2 保护造血系统免受辐射损伤。在本研究中，我们研究了酵母聚糖 A 的辐射防护作用的潜在机制。小鼠在电离辐射前 24 小时和 2 小时通过腹腔注射用酵母聚糖 A（50mg/kg，溶解在 NS 中）处理。通过流式细胞术检测骨髓细胞凋亡和白细胞介素 6（IL-6）、白细胞介素 12（IL-12）、粒细胞集落刺激因子（G-CSF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的水平。通过γ-H2AX 焦点测定法测定 DNA 损伤。此外，进行了 RNA 测序以鉴定差异表达基因（DEGs）。酵母聚糖 A 可保护骨髓细胞免受辐射诱导的凋亡，上调骨髓细胞中的 IL-6、IL-12、G-CSF 和 GM-CSF。酵母聚糖 A 还可保护细胞免受辐射诱导的 DNA 损伤。此外，RNA 测序分析显示，酵母聚糖 A 诱导了 131 个差异表达基因，这些基因参与了免疫系统过程和炎症反应的调节。DEGs 主要聚类在 18 个与免疫系统过程相关的 KEGG 途径中。酵母聚糖 A 可保护骨髓细胞免受辐射诱导的凋亡，并上调 IL-6、IL-12、G-CSF 和 GM-CSF。此外，酵母聚糖 A 还可能通过调节免疫系统过程和炎症反应发挥放射保护作用。这些结果为酵母聚糖 A 的放射保护作用提供了新的知识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/7a17307725c0/JCMM-22-2413-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/435350f39d01/JCMM-22-2413-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/cee3adf990a5/JCMM-22-2413-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/4c82496e31ae/JCMM-22-2413-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/76c5fc1bb22d/JCMM-22-2413-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/07255548a92b/JCMM-22-2413-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/7a17307725c0/JCMM-22-2413-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/435350f39d01/JCMM-22-2413-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/cee3adf990a5/JCMM-22-2413-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/4c82496e31ae/JCMM-22-2413-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/76c5fc1bb22d/JCMM-22-2413-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/07255548a92b/JCMM-22-2413-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2f/5867165/7a17307725c0/JCMM-22-2413-g006.jpg

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