College of Pharmacy, Beihua University, Jilin, Jilin 132013, China.
College of Pharmacy, Beihua University, Jilin, Jilin 132013, China.
Int Immunopharmacol. 2018 Mar;56:212-216. doi: 10.1016/j.intimp.2018.01.030. Epub 2018 Feb 3.
Our previous study found that Deproteinized Extract of Calf Blood (DECB) could protect the acute liver injury induced by carbon tetrachloride in mice, but the target-related transcription factors and their regulatory networks were not comprehensively studied. Based on the mRNA expression microarray data obtained in the previous study, the mRNA transcription factor regulatory networks were constructed by screening the transcription factors of differentially expressed genes and their corresponding target proteins, and the analysis on the functions and pathways of the regulatory network central nodes was performed. Eight genes Ltf, Tnf, Il6, Jun, Il12b, Stat3, Rel and Crem could regulate the inflammatory factors, and TNF signaling pathway and Jak-STAT signaling pathway might play an important role in the mechanism through which DECB protected the liver of mice. DECB can not only inhibit the apoptosis of hepatocytes, but also inhibit the inflammatory cytokines.
我们之前的研究发现，小牛血去蛋白提取物（DECB）可保护四氯化碳诱导的小鼠急性肝损伤，但未全面研究其相关的靶转录因子及其调控网络。基于之前研究中获得的 mRNA 表达微阵列数据，通过筛选差异表达基因的转录因子及其相应的靶蛋白，构建了 mRNA 转录因子调控网络，并对调控网络中心节点的功能和通路进行了分析。8 个基因 Ltf、Tnf、Il6、Jun、Il12b、Stat3、Rel 和 Crem 可调节炎症因子，TNF 信号通路和 Jak-STAT 信号通路可能在 DECB 保护小鼠肝的机制中发挥重要作用。DECB 不仅能抑制肝细胞凋亡，还能抑制炎症细胞因子。
