Andreev Dmitri E, Dmitriev Sergey E, Loughran Gary, Terenin Ilya M, Baranov Pavel V, Shatsky Ivan N
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Department of Biochemistry, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia.
Gene. 2018 Apr 20;651:174-182. doi: 10.1016/j.gene.2018.02.013. Epub 2018 Feb 4.
Eukaryotic cells evolved highly complex and accurate protein synthesis machinery that is finely tuned by various signaling pathways. Dysregulation of translation is a hallmark of many diseases, including cancer, and thus pharmacological approaches to modulate translation become very promising. While there has been much progress in our understanding of mammalian mRNA-specific translation control, surprisingly, relatively little is known about whether and how the protein components of the translation machinery shape translation of their own mRNAs. Here we analyze mammalian mRNAs encoding components of the translation initiation machinery for potential regulatory features such as 5'TOP motifs, TISU motifs, poor start codon nucleotide context and upstream open reading frames.
真核细胞进化出了高度复杂且精确的蛋白质合成机制，该机制由各种信号通路进行精细调节。翻译失调是包括癌症在内的许多疾病的一个标志，因此调节翻译的药理学方法变得非常有前景。尽管我们对哺乳动物mRNA特异性翻译控制的理解取得了很大进展，但令人惊讶的是，对于翻译机制的蛋白质成分是否以及如何影响其自身mRNA的翻译，我们知之甚少。在这里，我们分析编码翻译起始机制成分的哺乳动物mRNA，以寻找潜在的调控特征，如5'TOP基序、TISU基序、起始密码子核苷酸上下文不佳以及上游开放阅读框。
