School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China.
School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; Guangdong Engineering and Technology Research Center for Environmental Nanomaterials, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510642, PR China; Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
J Hazard Mater. 2018 May 5;349:51-59. doi: 10.1016/j.jhazmat.2018.01.046. Epub 2018 Feb 6.
Natural and cost-effective materials such as minerals can serve as supportive matrices to enhance biodegradation of polycyclic aromatic hydrocarbons (PAHs). In this study we evaluated and compared the regulatory role of two common soil minerals, i.e. kaolinite and quartz in phenanthrene (a model PAH) degradation by a PAH degrader Sphingomonas sp. GY2B and investigated the underlying mechanism. Overall kaolinite was more effective than quartz in promoting phenanthrene degradation and bacterial growth. And it was revealed that a more intimate association was established between GY2B and kaolinite. Si and O atoms on mineral surface were demonstrated to be involved in GY2B-mineral interaction. There was an higher polysaccharide/lipid content in the EPS (extracellular polymeric substances) secreted by GY2B on kaolinite than on quartz. Altogether, these results showed that differential bacterial growth, enzymatic activity, EPS composition as well as the interface interaction may explain the effects minerals have on PAH biodegradation. It was implicated that different interface interaction between different minerals and bacteria can affect microbial behavior, which ultimately results in different biodegradation efficiency.
天然且具有成本效益的材料，如矿物质，可以作为支持基质来增强多环芳烃（PAHs）的生物降解。在这项研究中，我们评估和比较了两种常见土壤矿物质，即高岭石和石英，在多环芳烃降解菌 Sphingomonas sp. GY2B 降解菲（一种模型 PAH）中的调控作用，并研究了其潜在机制。总体而言，高岭石比石英更能有效地促进菲的降解和细菌生长。研究表明，GY2B 与高岭石之间建立了更紧密的联系。矿物表面的 Si 和 O 原子被证明参与了 GY2B-矿物的相互作用。在 GY2B 分泌的 EPS（胞外聚合物）中，多糖/脂质含量在高岭石上高于石英。总的来说，这些结果表明，细菌生长、酶活性、EPS 组成以及界面相互作用的差异可能解释了矿物质对 PAH 生物降解的影响。这表明不同矿物质和细菌之间的不同界面相互作用会影响微生物的行为，最终导致不同的生物降解效率。
