Center for Reproductive Medicine, Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, The Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, China.
Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, China.
Gynecol Obstet Invest. 2018;83(3):241-246. doi: 10.1159/000486784. Epub 2018 Feb 7.
Polycystic ovary syndrome (PCOS) is a heterogeneous disorder characterized by chronic ovulatory dysfunction, hyperandrogenism, and polycystic ovaries. Wilms' tumor 1 (WT1) encoding a transcription factor involved in the differentiation of granulosa cells (GCs) regulates androgen receptor in the development of male genitalia. However, the expression pattern and possible role of WT1 in ovaries of PCOS patients are still unknown.
GCs from 95 PCOS patients (PCOS group) and 62 healthy controls (control group) were isolated. The expression of WT1 in GCs was quantified using the reverse transcription-polymerase chain reaction. The correlation between WT1 expression and clinical characteristics was evaluated in PCOS patients.
WT1 expression was increased in PCOS patients compared with the normal controls. The expression of WT1 was moderately correlated with testosterone (r = 0.334, p = 0.001) and luteinizing hormone (r = 0.357, p = 0.001) levels and the antral follicle counts (r = 0.337, p = 0.001).
Our study provided novel insights into the relationship between hyperandrogenism and polycystic ovaries of PCOS and WT1.
多囊卵巢综合征（PCOS）是一种异质性疾病，其特征为慢性排卵功能障碍、高雄激素血症和多囊卵巢。Wilms 肿瘤 1 基因（WT1）编码一种转录因子，参与颗粒细胞（GCs）的分化，调节雄激素受体在男性生殖器的发育。然而，WT1 在 PCOS 患者卵巢中的表达模式及其可能的作用尚不清楚。
从 95 例 PCOS 患者（PCOS 组）和 62 例健康对照（对照组）中分离出 GCs。采用逆转录-聚合酶链反应定量检测 GCs 中 WT1 的表达。评估 PCOS 患者 WT1 表达与临床特征的相关性。
与正常对照组相比，PCOS 患者的 WT1 表达增加。WT1 的表达与睾酮（r=0.334，p=0.001）和黄体生成素（r=0.357，p=0.001）水平以及窦卵泡计数（r=0.337，p=0.001）呈中度相关。
本研究为了解 PCOS 患者高雄激素血症和多囊卵巢与 WT1 之间的关系提供了新的见解。
