Negintaji Ahmad, Safahieh Alireza, Zolgharnein Hossein, Matroodi Soheila
Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
Toxicol Ind Health. 2018 Feb;34(2):119-127. doi: 10.1177/0748233717748099. Epub 2018 Feb 7.
Bisphenol A (BPA) is a known environmental endocrine-disrupting chemical that is widely used in plastics manufacturing. BPA enters in the aquatic environment mainly through urban and industrial sewage effluents, thereby posing a potential threat to organisms living in these ecosystems. This study was conducted to investigate the effect of BPA on VTG production with direct (sodium dodecyl sulfate-polyarylamide gel electrophoresis) and indirect (alkali-labile phosphate (ALP), total plasma calcium and protein) methods in immature male yellowfin seabream ( Acanthopagrus latus) as a marine fish model. Fish were randomly distributed into seven groups that were administered 1, 10, 50, and 100 µg g week of BPA and 2 µg gweek of 17β-estradiol (E2) over a period of 2 weeks. Solvent controls received olive oil, whereas controls were not injected. The fish were sampled on days 0, 7, and 14, and their blood plasma and liver were obtained. The results showed that the hepatosomatic index of all treated fish was elevated in comparison with controls. Direct and indirect indicators showed that fish VTG protein was induced by BPA and E2 exposure. The protein was found to have two bands with molecular weights around 210 and 190 KDa. ALP, total plasma calcium and protein levels were increased in dose- and time-dependent manners. The results of this study demonstrated that short-term exposure of yellowfin seabream to BPA induced adverse effects in the reproductive system of hermaphrodite fish.
双酚A（BPA）是一种已知的环境内分泌干扰化学物质，广泛应用于塑料制造。双酚A主要通过城市和工业污水排放进入水生环境，从而对生活在这些生态系统中的生物构成潜在威胁。本研究以未成熟雄性黄鳍鲷（Acanthopagrus latus）作为海洋鱼类模型，采用直接（十二烷基硫酸钠-聚丙烯酰胺凝胶电泳）和间接（碱不稳定磷酸盐（ALP）、血浆总钙和蛋白质）方法，研究双酚A对卵黄蛋白原（VTG）产生的影响。将鱼随机分为七组，在两周时间内分别给予1、10、50和100μg/g周的双酚A以及2μg/g周的17β-雌二醇（E2）。溶剂对照组接受橄榄油，而对照组不进行注射。在第0、7和14天对鱼进行采样，获取其血浆和肝脏。结果表明，与对照组相比，所有处理组鱼的肝体指数均升高。直接和间接指标显示，双酚A和E2暴露诱导了鱼的VTG蛋白。发现该蛋白有两条分子量约为210和190 kDa的条带。ALP、血浆总钙和蛋白质水平呈剂量和时间依赖性增加。本研究结果表明，黄鳍鲷短期暴露于双酚A会对雌雄同体鱼类的生殖系统产生不利影响。
