Köster Daniel, Wolbert Jens-Benjamin, Schulte Marcel S, Jochmann Maik A, Schmidt Torsten C
Instrumental Analytical Chemistry, University of Duisburg-Essen , Universitätsstrasse 5, 45141 Essen, Germany.
Biofilm Centre, University of Duisburg-Essen , Universitätsstrasse 5, 45141 Essen, Germany.
J Agric Food Chem. 2018 Feb 28;66(8):2015-2020. doi: 10.1021/acs.jafc.7b05212. Epub 2018 Feb 16.
The sugar replacement compound xylitol has gained increasing attention because of its use in many commercial food products, dental-hygiene articles, and pharmaceuticals. It can be classified by the origin of the raw material used for its production. The traditional "birch xylitol" is considered a premium product, in contrast to xylitol produced from agriculture byproducts such as corn husks or sugar-cane straw. Bulk stable-isotope analysis (BSIA) and compound-specific stable-isotope analysis (CSIA) by liquid-chromatography isotope-ratio mass spectrometry (LC-IRMS) of chewing-gum extracts were used to determine the δC isotope signatures for xylitol. These were applied to elucidate the original plant type the xylitol was produced from on the basis of differences in isotope-fractionation processes of photosynthetic CO fixation. For the LC-IRMS analysis, an organic-solvent-free extraction protocol and HPLC method for the separation of xylitol from different artificial sweeteners and sugar-replacement compounds was successfully developed and applied to the analysis of 21 samples of chewing gum, from which 18 could be clearly related to the raw-material plant class.
甜味替代化合物木糖醇因其在许多商业食品、口腔卫生用品和药品中的应用而受到越来越多的关注。它可以根据生产所用原材料的来源进行分类。与由玉米皮或甘蔗秸秆等农业副产品生产的木糖醇相比，传统的“桦木木糖醇”被视为优质产品。通过液相色谱同位素比率质谱法（LC-IRMS）对口香糖提取物进行批量稳定同位素分析（BSIA）和化合物特异性稳定同位素分析（CSIA），以确定木糖醇的δC同位素特征。基于光合CO固定同位素分馏过程的差异，这些特征被用于阐明木糖醇所源自的原始植物类型。对于LC-IRMS分析，成功开发了一种无有机溶剂的提取方案和用于从不同人工甜味剂和甜味替代化合物中分离木糖醇的HPLC方法，并将其应用于21份口香糖样品的分析，其中18份可以明确与原材料植物类别相关。
