Li Kun, Chen Zhiting, Qin Yanwen, Wei Yongxiang
Department of Otolaryngology, Beijing AnZhen Hospital, Capital Medical University, Beijing, China.
Medicine (Baltimore). 2018 Feb;97(6):e9813. doi: 10.1097/MD.0000000000009813.
The early prediction of atherosclerosis (AS) is important in the management of obstructive sleep apnea patients (OSA). MicroRNA (miRNA) plays a vital role in the evolution of OSA and AS. Its differential expression may therefore serve as a diagnostic and prognostic biomarker of AS in OSA. The aim of this study was to identify specific serum miRNAs that could serve as a novel screening signature of AS in OSA patients. The specificity and sensitivity of these miRNAs in the early diagnosis of AS in OSA patients were then determined.The 128 participants in this study underwent maximum carotid intima-media thickness (CIMT) measurements and polysomnography and were divided into 4 groups: 27 healthy volunteers with normal max-CIMT, 31 healthy volunteers with increased max-CIMT, 35 OSA patients with normal max-CIMT, and 35 OSA patients with iCIMT. MiRNA was extracted from the 12 participants' serum (3 participants each groups) and used to establish miRNA libraries for deep sequencing. A total of 116 participants were quantified by qRT- PCR. Correlations between differential expression of miRNAs and CIMT were assessed using the Spearman correlation coefficient. Our study was approved by the Ethics Committee of our hospital and was conducted in line with the Helsinki Declaration.MiR-664a-3p expression was quantified by qRT-PCR. Correlations between miR-664a-3p expression and CIMT were assessed using the Spearman correlation coefficient. The results showed that the miR-664a-3p was downregulated in the OSA, OSA with iCMIT, and nCIMT groups compared with the control group.The demonstrated potential of circulating miR-664a-3p as a noninvasive marker of AS in essential OSA patients should be confirmed in further studies.
动脉粥样硬化（AS）的早期预测对于阻塞性睡眠呼吸暂停患者（OSA）的管理至关重要。微小RNA（miRNA）在OSA和AS的发展中起着至关重要的作用。因此，其差异表达可能作为OSA中AS的诊断和预后生物标志物。本研究的目的是鉴定可作为OSA患者AS新型筛查标志物的特定血清miRNA。然后确定这些miRNA在OSA患者AS早期诊断中的特异性和敏感性。本研究中的128名参与者接受了最大颈动脉内膜中层厚度（CIMT）测量和多导睡眠图检查，并分为4组：27名最大CIMT正常的健康志愿者，31名最大CIMT增加的健康志愿者，35名最大CIMT正常的OSA患者，以及35名iCIMT的OSA患者。从12名参与者的血清中提取miRNA（每组3名参与者），并用于建立miRNA文库进行深度测序。共有116名参与者通过qRT-PCR进行定量。使用Spearman相关系数评估miRNA差异表达与CIMT之间的相关性。我们的研究得到了我院伦理委员会的批准，并按照《赫尔辛基宣言》进行。通过qRT-PCR对miR-664a-3p表达进行定量。使用Spearman相关系数评估miR-664a-3p表达与CIMT之间的相关性。结果显示，与对照组相比，miR-664a-3p在OSA、iCMIT的OSA和nCIMT组中表达下调。循环miR-664a-3p作为原发性OSA患者AS的非侵入性标志物的潜在作用应在进一步研究中得到证实。
