Bi Renzhe, Balasundaram Ghayathri, Jeon Seungwan, Tay Hui Chien, Pu Yang, Li Xiuting, Moothanchery Mohesh, Kim Chulhong, Olivo Malini
Singapore Bioimaging Consortium, Singapore.
Department of Creative IT Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.
J Biophotonics. 2018 Oct;11(10):e201700327. doi: 10.1002/jbio.201700327. Epub 2018 May 9.
The use of an optical resolution photoacoustic microscopy (OR-PAM) system to evaluate the vascular disruptive effect of combretastatin A4 Phosphate (CA4P) on a murine orthotopic glioma with intact skull is described here. Second generation optical-resolution photoacoustic microscopy scanner with a 532 nm pulsed diode-pumped solid-state laser that specifically matches the absorption maximum of hemoglobin in tissues was used to image orthotopic glioma inoculated in mouse brain. Two-dimensional maps of brain vasculature with a lateral resolution of 5 μm and a depth of 700 μm at a field of view 5 × 4 mm were acquired on normal brain and glioma brain. Longitudinal imaging of the brain pre- and post-administration of CA4P, a FDA approved drug for solid tumors, enabled the monitoring of hemodynamic changes in tumor vasculature revealing the well documented vascular shutdown and recovery associated with this drug. Our study marks the beginning of potential prospects of this technology as an imaging tool for preclinical and clinical study of pathologies characterized by changes in the vasculature.
本文描述了使用光学分辨率光声显微镜（OR-PAM）系统评估磷酸柯里拉京A4（CA4P）对颅骨完整的小鼠原位胶质瘤的血管破坏作用。使用第二代光学分辨率光声显微镜扫描仪，其配备532 nm脉冲二极管泵浦固态激光器，该激光器与组织中血红蛋白的最大吸收峰特异性匹配，用于对接种于小鼠脑内的原位胶质瘤进行成像。在正常脑和胶质瘤脑上获取了二维脑脉管系统图，横向分辨率为5μm，深度为700μm，视野为5×4 mm。对已获美国食品药品监督管理局批准用于实体瘤的CA4P给药前后的脑进行纵向成像，能够监测肿瘤脉管系统中的血流动力学变化，揭示了与该药物相关的已充分记录的血管关闭和恢复情况。我们的研究标志着这项技术作为一种成像工具用于以脉管系统变化为特征的病理学临床前和临床研究的潜在前景的开端。
