Hossain Sabrina, De Silva Benthotage Chamara Jayasankha, Wimalasena Sudu Hakuruge Madusha Pramud, Pathirana Hansani Nilupama Kumari Senarath, Dahanayake Pasan Sepala, Heo Gang-Joon
Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University , Cheongju, Korea.
Microb Drug Resist. 2018 Oct;24(8):1217-1225. doi: 10.1089/mdr.2017.0388. Epub 2018 Feb 8.
Aeromonas spp. are opportunistic pathogens related to multiple infectious diseases in ornamental fishes. In the present study, the antimicrobial susceptibility, resistance genes, and integrons of 65 goldfish-borne Aeromonas spp. were evaluated. The isolates were identified as A. hydrophila (n = 30), A. veronii (n = 32), and A. punctata (n = 3) by gyrB sequencing. The antimicrobial susceptibility testing of the isolates designated that most of the isolates were resistant to amoxicillin (100.00%), nalidixic acid (100.00%), ampicillin (98.46%), tetracycline (92.31%), rifampicin (86.15%), and cephalothin (61.54%) and each of the isolates showed multiple antimicrobial resistance phenotype (resistant to ≥3 classes of antimicrobials). PCR amplification of antimicrobial resistance genes revealed that the plasmid-mediated quinolone resistance gene, qnrS, was the most prevalent (73.85%) among the isolates. The other antimicrobial resistance genes were detected in the following proportions: qnrB (26.15%), aac(6')-Ib-cr (4.60%), tetA (16.92%), tetE (21.54%), aac(6')-Ib (29.23%), and aphAI-IAB (7.69%). The IntI gene was found in 64.62% isolates, and four class 1 integron gene cassette profiles (incomplete dfrA1, catB3-aadA1, dfrA1-orfC, and qacE2-orfD) were identified. These data suggest that goldfish-borne Aeromonas spp. serve as a reservoir of antimicrobial resistance genes and class 1 integrons.
气单胞菌属是与观赏鱼多种传染病相关的机会致病菌。在本研究中，对65株金鱼源气单胞菌属的抗菌药敏性、耐药基因和整合子进行了评估。通过gyrB测序将分离株鉴定为嗜水气单胞菌（n = 30）、维氏气单胞菌（n = 32）和点状气单胞菌（n = 3）。分离株的抗菌药敏试验表明，大多数分离株对阿莫西林（100.00%）、萘啶酸（100.00%）、氨苄西林（98.46%）、四环素（92.31%）、利福平（86.15%）和头孢噻吩（61.54%）耐药，并且每个分离株都表现出多重抗菌耐药表型（对≥3类抗菌药物耐药）。抗菌耐药基因的PCR扩增显示，质粒介导的喹诺酮耐药基因qnrS在分离株中最为普遍（73.85%）。其他抗菌耐药基因的检出比例如下：qnrB（26.15%）、aac(6')-Ib-cr（4.60%）、tetA（16.92%）、tetE（21.54%）、aac(6')-Ib（29.23%）和aphAI-IAB（7.69%）。在64.62%的分离株中发现了IntI基因，并鉴定出四种1类整合子基因盒图谱（不完整的dfrA1、catB3-aadA1、dfrA1-orfC和qacE2-orfD）。这些数据表明，金鱼源气单胞菌属是抗菌耐药基因和1类整合子的储存库。
