Wu Jinlong, Deng Wenjun, Lin Dingbo, Deng Xiuxin, Ma Zhaocheng
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University , Wuhan 430070, China.
Department of Nutritional Sciences, Oklahoma State University , 419 Human Sciences, Stillwater, Oklahoma 74078, United States.
J Agric Food Chem. 2018 Feb 28;66(8):1964-1973. doi: 10.1021/acs.jafc.7b05722. Epub 2018 Feb 16.
The inherent allergens of citrus fruits, such as Cit s 1, Cit s 2, Cit s 3 can cause allergic reactions. A better understanding of the genetic factors (cultivar to cultivar) affecting the allergenic potential of citrus fruits would be beneficial for further identification of hypoallergenic genotypes. In the present study, an immunoblotting quantification approach was adopted to assess the potential allergenicity of 21 citrus cultivars, including nine subgroups (tangerine, satsuma, orange, pummelo, grapefruit, lemon, kumquat, tangor, and tangelo). To prepare highly sensitive and specific rabbit polyclonal antibodies, antigenicity of purified rCit s 1.01, rCit s 2.01, and rCit s 3.01 peptides were enhanced with high epitope density in a single protein molecule. The data integration of three citrus allergen quantifications demonstrated that the four pummelo cultivars (Kao Phuang Pummelo, Wanbai Pummelo, Shatian Pummelo, and Guanxi Pummelo) were potential hypoallergenic, compared with other 8 subgroups. Moreover, the immunological analyses with sera of allergic subjects revealed that Shatian Pummelo and Guanxi Pummelo showed the lowest immunoreactivity in 8 representative citrus cultivars. These potential hypoallergenic genotypes are of great significance to not only allergic consumers but also citrus breeders in the genetic improvement of hypoallergenic citrus as breeding resources.
柑橘类水果的固有过敏原，如柑橘过敏原1（Cit s 1）、柑橘过敏原2（Cit s 2）、柑橘过敏原3（Cit s 3），可引起过敏反应。更好地了解影响柑橘类水果致敏潜力的遗传因素（品种间），将有助于进一步鉴定低致敏性基因型。在本研究中，采用免疫印迹定量方法评估了21个柑橘品种的潜在致敏性，这些品种包括9个亚组（蜜橘、温州蜜柑、橙子、柚、葡萄柚、柠檬、金橘、橘橙和橘柚）。为制备高灵敏度和特异性的兔多克隆抗体，在单个蛋白质分子中通过高表位密度增强了纯化的重组柑橘过敏原1.01（rCit s 1.01）、重组柑橘过敏原2.01（rCit s 2.01）和重组柑橘过敏原3.01（rCit s 3.01）肽的抗原性。三种柑橘过敏原定量的数据整合表明，与其他8个亚组相比，四个柚品种（高磅柚、晚白柚、沙田柚和琯溪蜜柚）具有潜在的低致敏性。此外，对过敏受试者血清的免疫学分析表明，在8个代表性柑橘品种中，沙田柚和琯溪蜜柚的免疫反应性最低。这些潜在的低致敏性基因型不仅对过敏消费者具有重要意义，而且对柑橘育种者在利用低致敏性柑橘作为育种资源进行遗传改良方面也具有重要意义。
