Nag A C, Cheng M
Biochem Biophys Res Commun. 1986 Jun 13;137(2):855-62. doi: 10.1016/0006-291x(86)91158-7.
Myosin isozyme pattern in adult rat cardiac ventricular muscle cells in long-term culture was investigated. The myosin isozymes profile of cultured cardiac myocytes underwent a change in a serum-containing medium from two weeks onward, showing an embryonic rat ventricular myosin isozymes pattern that contained predominant isozyme V3. When adult cardiac myocytes were grown in a serum-containing medium supplemented with T4, these cells contained a predominant V1 band whose electrophoretic mobility and Ca2+-ATPase activity were comparable to those of the adult rat ventricle in vivo. This study has demonstrated that the adult cardiac ventricular muscle cells in long-term culture contain a predominant myosin isozyme V3 unlike their counterparts in vivo. Supplemented T4 modulated the embryonic type isozyme V3 to the adult type V1.
研究了长期培养的成年大鼠心室肌细胞中的肌球蛋白同工酶模式。培养的心肌细胞的肌球蛋白同工酶谱在含血清培养基中从两周起发生变化，呈现出胚胎大鼠心室肌球蛋白同工酶模式，其中主要同工酶为V3。当成年心肌细胞在添加了T4的含血清培养基中生长时，这些细胞含有主要的V1条带，其电泳迁移率和Ca2+ - ATP酶活性与成年大鼠体内心室的相当。本研究表明，长期培养的成年心室肌细胞含有主要的肌球蛋白同工酶V3，这与它们在体内的对应物不同。添加的T4将胚胎型同工酶V3调节为成年型V1。
