抗氧化剂和/或表观遗传酶抑制剂的组合能够在环境空气中提高小鼠骨髓造血干细胞的采集量。
Combinations of antioxidants and/or of epigenetic enzyme inhibitors allow for enhanced collection of mouse bone marrow hematopoietic stem cells in ambient air.
作者信息
Cai Qingchun, Capitano Maegan, Huang Xinxin, Guo Bin, Cooper Scott, Broxmeyer Hal E
机构信息
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.
出版信息
Blood Cells Mol Dis. 2018 Jul;71:23-28. doi: 10.1016/j.bcmd.2018.01.005. Epub 2018 Feb 13.
Abstract
Hematopoietic cell transplantation (HCT) is a treatment for malignant and non-malignant disorders. However, sometimes the numbers of donor hematopoietic stem cells (HSC) are limiting, which can compromise the success of HCT. We recently published that collection and processing of mouse bone marrow (BM) and human cord blood cells in a hypoxic atmosphere of 3% O or in ambient air (~21% O) in the presence of cyclosporine A yields increased numbers of HSC. We now show that collection and processing of mouse BM cells in ambient air in the presence of specific combinations of anti-oxidants and/or inhibitors of epigenetic enzymes can also enhance the collection of HSC, information of potential relevance for enhanced efficacy of HCT.
摘要
造血细胞移植（HCT）是一种针对恶性和非恶性疾病的治疗方法。然而，有时供体造血干细胞（HSC）的数量有限，这可能会影响HCT的成功率。我们最近发表的研究表明，在3%氧气的低氧环境或空气中（约21%氧气），在环孢素A存在的情况下收集和处理小鼠骨髓（BM）和人脐带血细胞，可增加HSC的数量。我们现在表明，在抗氧化剂和/或表观遗传酶抑制剂的特定组合存在的情况下，在空气中收集和处理小鼠BM细胞，也可以提高HSC的收集量，这一信息对于提高HCT的疗效可能具有潜在相关性。
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本文引用的文献
1
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Nat Med. 2017 Apr;23(4):424-428. doi: 10.1038/nm.4298. Epub 2017 Mar 6.
2
Enhancing the efficacy of engraftment of cord blood for hematopoietic cell transplantation.提高脐带血用于造血细胞移植的植入疗效。
Transfus Apher Sci. 2016 Jun;54(3):364-72. doi: 10.1016/j.transci.2016.05.013. Epub 2016 May 12.
3
Enhancing Hematopoietic Stem Cell Transplantation Efficacy by Mitigating Oxygen Shock.通过减轻氧休克提高造血干细胞移植疗效
Cell. 2015 Jun 18;161(7):1553-65. doi: 10.1016/j.cell.2015.04.054. Epub 2015 Jun 11.
4
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Curr Opin Hematol. 2015 Jul;22(4):273-8. doi: 10.1097/MOH.0000000000000144.
5
Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.N-乙酰半胱氨酸和抗坏血酸-2-磷酸对人间充质干细胞抗有丝分裂性细胞死亡、坏死性凋亡和凋亡的协同保护作用。
Sci Rep. 2015 Apr 24;5:9819. doi: 10.1038/srep09819.
6
Mild Heat Treatment Primes Human CD34(+) Cord Blood Cells for Migration Toward SDF-1α and Enhances Engraftment in an NSG Mouse Model.轻度热处理使人类CD34(+)脐血细胞对SDF-1α产生趋化性并增强在NSG小鼠模型中的植入。
Stem Cells. 2015 Jun;33(6):1975-84. doi: 10.1002/stem.1988.
7
Antioxidant N-acetyl-L-cysteine increases engraftment of human hematopoietic stem cells in immune-deficient mice.抗氧化剂N-乙酰-L-半胱氨酸可提高人造血干细胞在免疫缺陷小鼠体内的植入率。
Blood. 2014 Nov 13;124(20):e45-8. doi: 10.1182/blood-2014-03-559369. Epub 2014 Oct 6.
8
Hematopoietic stem cell niche maintenance during homeostasis and regeneration.造血干细胞龛在稳态和再生中的维持。
Nat Med. 2014 Aug;20(8):833-46. doi: 10.1038/nm.3647.
9
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Nature. 2014 Apr 10;508(7495):269-73. doi: 10.1038/nature13034. Epub 2014 Mar 2.
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