São Leopoldo Mandic School of Dentistry, Campinas, Brazil.
Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Oral Dis. 2018 Sep;24(6):957-963. doi: 10.1111/odi.12843. Epub 2018 May 30.
To investigate whether saliva formulations with reduced calcium (Ca) and inorganic phosphorous (Pi) concentration would affect dental erosion caused by hydrochloric acid (HCl).
Enamel and root dentine bovine slabs were embedded, polished, and measured for surface Knoop microhardness (SMH). After reference areas were created, specimens were exposed to HCl solution (0.01 M; pH 2; 120 s) and immersed in artificial salivas (6 hr) containing three different Ca/Pi concentrations (n = 15), which simulate serum conditions of normo-, mild, or severe hypocalcemia. The control group was immersed in Ca/Pi-free saliva. The study protocol was carried out 2×/day for 5 days. Surface loss of enamel and root dentine was assessed using an optical profilometer, and SMH was remeasured for enamel.
One-way analysis of variance (p < .001) and Tukey's test showed that enamel loss in groups subjected to artificial salivas that simulated mild or severe hypocalcemia did not differ from that resembling normocalcemia. %SMH was lower when saliva was mildly and normally concentrated in Ca/Pi (p < .001). Root dentine loss was higher in saliva simulating severe hypocalcemia than in those referring to mild, hypo-, and normocalcemia.
Depending on the dental substrate, salivary formulations resembling serum hypocalcemia affected surface loss due to erosion and rehardening thereof.
研究降低钙（Ca）和无机磷（Pi）浓度的唾液配方是否会影响盐酸（HCl）引起的牙齿腐蚀。
将牛釉质和根牙本质板嵌入、抛光并测量表面努普微硬度（SMH）。在创建参考区域后，将标本暴露于 HCl 溶液（0.01 M；pH 2；120 s）并浸入含有三种不同 Ca/Pi 浓度（n = 15）的人工唾液中，这模拟了正常、轻度或重度低钙血症的血清条件。对照组浸入无 Ca/Pi 的唾液中。该研究方案每天进行 2 次，共 5 天。使用光学轮廓仪评估釉质和根牙本质的表面损失，并重新测量釉质的 SMH。
单因素方差分析（p <.001）和 Tukey 检验表明，模拟轻度或重度低钙血症的人工唾液组的釉质损失与正常钙血症相似。当 Ca/Pi 浓度轻度和正常时，%SMH 较低（p <.001）。模拟严重低钙血症的唾液中根牙本质的损失高于模拟轻度、低钙血症和正常钙血症的唾液。
根据牙齿基质的不同，模拟血清低钙血症的唾液配方会影响因腐蚀和再硬化引起的表面损失。
