顺铂通过下调 XIAP 诱导肝癌 LM3 细胞凋亡。

Cisplatin induces apoptosis of hepatocellular carcinoma LM3 cells via down-regulation of XIAP.

机构信息

Department of Hepatopancreaticobiliary Surgery and Laparoscopic Minimally Invasive Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, P.R. China.

出版信息

Eur Rev Med Pharmacol Sci. 2018 Jan;22(2):382-387. doi: 10.26355/eurrev_201801_14183.

DOI:10.26355/eurrev_201801_14183

PMID:29424923

Abstract

OBJECTIVE

Cisplatin is an important anti-cancer drug. However, the molecular mechanism of cisplatin on inhibition of the proliferation of liver cancer cells is unclear. Thus, we aimed to investigate the regulatory role of cisplatin on the growth and apoptosis of hepatoma LM3 cells.

MATERIALS AND METHODS

LM3 cells were treated with cisplatin (2 μmol/L) for 48 h. MTT assay, flow cytometry, and caspase-3 activity assay were used to detect the growth, proliferation and apoptosis of LM3 cells. Western blot was applied to detect the expression of the inhibitor of apoptosis protein, XIAP. siRNA was used to knockdown the level of XIAP followed by cisplatin (2 μmol/L) treatment, and then the apoptosis of LM3 cells was measured.

RESULTS

The treatment of cisplatin significantly inhibited the growth but induced the apoptosis of LM3 cells. Cisplatin also downregulated the expression of XIAP. The downregulation of XIAP by using siRNA enhanced the apoptosis of LM3 cells induced by cisplatin.

CONCLUSIONS

Downregulation of XIAP enhanced the proapoptotic effect of cisplatin on LM3 cells, suggesting that XIAP might be used as a potential target in the treatment of liver cancer.

摘要

目的

顺铂是一种重要的抗癌药物。然而，顺铂抑制肝癌细胞增殖的分子机制尚不清楚。因此，我们旨在研究顺铂对肝癌 LM3 细胞生长和凋亡的调节作用。

材料与方法

用顺铂（2μmol/L）处理 LM3 细胞 48 小时。采用 MTT 法、流式细胞术和 caspase-3 活性测定法检测 LM3 细胞的生长、增殖和凋亡。Western blot 法检测凋亡抑制蛋白 XIAP 的表达。用 siRNA 敲低 XIAP 水平，然后用顺铂（2μmol/L）处理，再检测 LM3 细胞的凋亡。