在神经损伤中通过磁共振成像监测Toll样受体4激活增强的巨噬细胞募集。
Monitoring of macrophage recruitment enhanced by Toll-like receptor 4 activation with MR imaging in nerve injury.
作者信息
Chen Mei-Wei, Zhang Xiang, Lu Lie-Jing, Zhang Fang, Duan Xiao-Hui, Zheng Chu-Shan, Chen Yue-Yao, Shen Jun
机构信息
Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No.107 Yanjiang Road West, Guangzhou, 510120, China.
出版信息
Muscle Nerve. 2018 Feb 9. doi: 10.1002/mus.26097.
INTRODUCTION
Macrophage recruitment is critical for nerve regeneration after an injury. The aim of this study was to investigate whether ultrasmall superparamagnetic iron oxide (USPIO) nanoparticle-based MRI could be used to monitor the enhanced macrophage recruitment by Toll-like receptor 4 (TLR4) activation in nerve injury.
METHODS
Rats received intraperitoneal injections of either lipopolysaccharide (LPS) or phosphate buffered saline (PBS) or no injection (controls) after a sciatic nerve crush injury. After intravenous injection of the USPIOs (LPS and PBS groups) or PBS (control group), MRI was performed and correlated with histological findings.
RESULTS
LPS group showed more remarkable hypointense signals on T2*-weighted imaging and lower T2 values in the crushed nerves than PBS group. The hypointense signal areas were associated with an enhanced recruitment of iron-loaded macrophages to the injured nerves.
DISCUSSION
USPIO-enhanced MRI can be used to monitor the enhanced macrophage recruitment by means of TLR4 signal pathway activation in nerve injury. Muscle Nerve, 2018.
引言
巨噬细胞募集对于损伤后的神经再生至关重要。本研究的目的是调查基于超小超顺磁性氧化铁（USPIO）纳米颗粒的磁共振成像（MRI）是否可用于监测在神经损伤中通过Toll样受体4（TLR4）激活而增强的巨噬细胞募集。
方法
大鼠在坐骨神经挤压伤后接受腹腔注射脂多糖（LPS）或磷酸盐缓冲盐水（PBS）或不注射（对照组）。在静脉注射USPIO（LPS组和PBS组）或PBS（对照组）后，进行MRI检查并与组织学结果相关联。
结果
与PBS组相比，LPS组在T2 *加权成像上显示出更明显的低信号，并且在挤压神经中的T2值更低。低信号区域与铁负载巨噬细胞向损伤神经的募集增强相关。
讨论
USPIO增强MRI可用于通过神经损伤中TLR4信号通路激活来监测增强的巨噬细胞募集。《肌肉与神经》，2018年
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