Youdim M B, Sills M A, Heydorn W E, Creed G J, Jacobowitz D M
J Neurochem. 1986 Sep;47(3):794-9. doi: 10.1111/j.1471-4159.1986.tb00681.x.
Young rats (21 days old) made nutritionally iron deficient, by feeding them a semisynthetic diet containing skimmed milk for 5 weeks, had significantly lowered hemoglobin levels (5.2 +/- 4 g/100 ml). The nonheme iron content in caudate nucleus was decreased by 47%. The behavioral response of iron-deficient rats to apomorphine (2 mg/kg) and the density of 3,4-dihydroxyphenylethylamine (dopamine) D2 receptors, as measured by [3H]spiperone binding in caudate nucleus, were significantly reduced by 70 and 53%, respectively. The possibility that nutritional iron deficiency may affect protein content in brain was investigated by measuring the apparent concentration of proteins in caudate nucleus and nucleus accumbens from iron-deficient and control animals using two-dimensional gel electrophoresis. The data indicate that iron deficiency can affect content in these two brain regions. Significant changes in the content of 10 proteins were noted in the caudate nucleus and nucleus accumbens in iron-deficient rats. The albumin level was significantly increased in both regions studied, whereas the neuron-specific enolase level was increased in the nucleus accumbens and the glial fibrillary acidic protein level was reduced in the caudate nucleus. The significance of these protein content changes, as well as a reduction in content of a 94-kilodalton protein (a molecular size similar to that of the D2 dopamine receptor), remains to be established.
通过给21日龄的幼鼠喂食含脱脂牛奶的半合成饲料5周，使其营养性缺铁，结果发现这些幼鼠的血红蛋白水平显著降低（5.2±4克/100毫升）。尾状核中的非血红素铁含量下降了47%。缺铁大鼠对阿扑吗啡（2毫克/千克）的行为反应以及通过尾状核中[3H]螺哌隆结合测定的3,4-二羟基苯乙胺（多巴胺）D2受体密度，分别显著降低了70%和53%。通过二维凝胶电泳测量缺铁和对照动物尾状核和伏隔核中蛋白质的表观浓度，研究了营养性缺铁可能影响脑内蛋白质含量的可能性。数据表明缺铁会影响这两个脑区的蛋白质含量。缺铁大鼠的尾状核和伏隔核中有10种蛋白质的含量发生了显著变化。在研究的两个区域中，白蛋白水平均显著升高，而伏隔核中神经元特异性烯醇化酶水平升高，尾状核中胶质纤维酸性蛋白水平降低。这些蛋白质含量变化的意义以及一种94千道尔顿蛋白质（分子大小与D2多巴胺受体相似）含量的降低，仍有待确定。
