Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, PR China.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, PR China.
Talanta. 2018 May 1;181:24-31. doi: 10.1016/j.talanta.2017.12.049. Epub 2017 Dec 19.
In this work, we develop a fluorescent molecular beacon based on the DNA-templated silver nanoclusters (DNA-Ag NCs). The skillfully designed molecular beacon can be conveniently used for detection of diverse virulence genes as long as the corresponding recognition sequences are embedded. Importantly, the constructed detection system allows simultaneous detection of multiple nucleic acids, which is attributed to non-overlapping emission spectra of the as-synthesized silver nanoclusters. Based on the target-induced fluorescence enhancement, three infectious disease-related genes HIV, H1N1, and H5N1 are detected, and the corresponding detection limits are 3.53, 0.12 and 3.95nM, respectively. This design allows specific, versatile and simultaneous detection of diverse targets with easy operation and low cost.
在这项工作中，我们开发了一种基于 DNA 模板银纳米簇（DNA-Ag NCs）的荧光分子信标。巧妙设计的分子信标可以方便地用于检测多种毒力基因，只要嵌入相应的识别序列。重要的是，所构建的检测系统允许同时检测多种核酸，这归因于所合成的银纳米簇的非重叠发射光谱。基于目标诱导的荧光增强，检测了三种与传染病相关的基因 HIV、H1N1 和 H5N1，相应的检测限分别为 3.53、0.12 和 3.95nM。这种设计允许通过简单的操作和低成本实现对多种靶标的特异性、多功能和同时检测。
