Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Talanta. 2018 May 1;181:326-332. doi: 10.1016/j.talanta.2018.01.043. Epub 2018 Jan 31.
Nylon 6 nanofibers were tested for their ability to serve as a sorbent for solid phase extraction (SPE). The regular nanostructure providing a great sorption area and amidic functionality should lead to the assumption that nylon 6 nanofibers could be used as a novel sorbent with great potential for sample pre-treatment. However, due to the substantial differences between classical particle sorbents used for solid phase extraction and nanofibers, it is necessary to evaluate this novel approach. This article describes three types of laboratory fabricated nylon 6 nanofibers with different surface density (5.04gm, 3.90gm and 0.75gm) and corresponding surface areas for solid phase extraction of several groups of compounds with different structural and physicochemical properties (parabens, steroids, flavonoids and pesticides). The nanofibers were created by needleless electrospinning. Extraction columns were manually packed in classic 1- or 3-mL plastic syringe cartridges with 26-30mg of nanofibers and the column bed was sealed with polypropylene frits. The SPE procedure followed a typical five-step protocol and the collected eluates were analyzed by HPLC with UV detection. Extraction recovery was used as a parameter to evaluate the behavior of the analytes within the SPE process. Under this set condition, the recovery of the SPE process ranged from 23.1% to 125.8%. SPE showed good repeatability (0.58-11.87% RSD) and inter-day reproducibility (3.86-9.79% RSD). The achieved results were compared with SPE using a classic particle sorbent column. Good mechanical and chemical stability of nanofibers was proved. Scanning electron microscope was used for the evaluation of morphological changes in nanostructure. Nylon 6 nanofibers proved being a cost-effective sorbent for repeated use in SPE. Nylon 6 nanofibers have great potential in miniaturized SPE enabling users to overcome troubles with high back-pressure.
尼龙 6 纳米纤维被测试其作为固相萃取（SPE）吸附剂的能力。规则的纳米结构提供了巨大的吸附面积和酰胺官能团，这应该使人们假设尼龙 6 纳米纤维可以作为一种具有巨大潜力的新型吸附剂，用于样品预处理。然而，由于经典的固相萃取用粒子吸附剂和纳米纤维之间存在显著差异，有必要对这种新方法进行评估。本文描述了三种不同表面密度（5.04gm、3.90gm 和 0.75gm）和相应比表面积的实验室制备的尼龙 6 纳米纤维，用于固相萃取几类具有不同结构和物理化学性质的化合物（对羟基苯甲酸酯、甾体、类黄酮和农药）。纳米纤维是通过无针电纺制成的。萃取柱通过手动填充在经典的 1 或 3ml 塑料注射器筒中，填充 26-30mg 纳米纤维，柱床用聚丙烯滤片密封。SPE 过程遵循典型的五步程序，收集的洗脱液通过 HPLC 进行分析，采用紫外检测。萃取回收率作为评价分析物在 SPE 过程中行为的参数。在这种设定条件下，SPE 过程的回收率在 23.1%至 125.8%之间。SPE 显示出良好的重复性（0.58-11.87%RSD）和日间重现性（3.86-9.79%RSD）。将所得到的结果与使用经典粒子吸附剂柱的 SPE 进行比较。证明纳米纤维具有良好的机械和化学稳定性。扫描电子显微镜用于评估纳米结构的形态变化。尼龙 6 纳米纤维被证明是一种具有成本效益的吸附剂，可用于 SPE 的重复使用。尼龙 6 纳米纤维在微型 SPE 中具有很大的潜力，使使用者能够克服高压降带来的麻烦。
