Alayande Abayomi Babatunde, Aung Mar Mar, Kim In S
Global Desalination Research Center (GDRC), School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju, 61005, South Korea.
Department of Biotechnology, Mandalay Technological University (MTU), Patheingyi, Mandalay Region, Republic of the Union of Myanmar.
Curr Microbiol. 2018 Jul;75(7):787-793. doi: 10.1007/s00284-018-1449-5. Epub 2018 Feb 9.
Bacteria, when adhered to a substratum, can form biofilms. Nevertheless, many factors dictate biofilm formation and virulence factor production, including a response by the bacteria to their surroundings. This system is referred to as Quorum sensing (QS) also known as cell-cell communication. Pseudomonas aeruginosa is an infection causing agent in immune-compromised patients, it uses acyl-homoserine lactone (AHL) to coordinate its QS systems. In this work, the connection between some members of AHL produced by P. aeruginosa PAO1 and its biofilm development and the production of virulence factor was investigated. It was discovered that N-butanoyl-homoserine lactone (C4-HSL) and N-hexanoyl-L-homoserine lactone (C6-HSL) perform a more consequential and eminent function in the biofilm maturation and virulence factor production while N-(3-oxododecanoyl)-L-homoserine lactone (3OC12-HSL) plays a role in biofilm initiation. Because QS has been reported to be required for biofilm development and pathogenesis of P. aeruginosa, the results of this work have great importance and significance for the design of strategies for the control and prevention of biofilms.
细菌附着在基质上时会形成生物膜。然而，许多因素决定生物膜的形成和毒力因子的产生，包括细菌对周围环境的反应。这个系统被称为群体感应（QS），也称为细胞间通讯。铜绿假单胞菌是免疫功能低下患者的感染病原体，它利用酰基高丝氨酸内酯（AHL）来协调其群体感应系统。在这项工作中，研究了铜绿假单胞菌PAO1产生的一些AHL成员与其生物膜发育和毒力因子产生之间的联系。研究发现，N-丁酰高丝氨酸内酯（C4-HSL）和N-己酰-L-高丝氨酸内酯（C6-HSL）在生物膜成熟和毒力因子产生中发挥更重要和显著的作用，而N-（3-氧代十二酰基）-L-高丝氨酸内酯（3OC12-HSL）在生物膜起始中起作用。由于据报道群体感应是铜绿假单胞菌生物膜发育和发病机制所必需的，这项工作的结果对于设计控制和预防生物膜的策略具有重要意义。
