Leo-Penu C L O, Picard J, Fitzpatrick L A, Parker A J
Kupang State Agricultural Polytechnic, East Nusa Tenggara, Indonesia.
School of Veterinary and Biomedical Sciences, James Cook University, Townsville, Qld, Australia.
J Anim Physiol Anim Nutr (Berl). 2018 Jun;102(3):670-678. doi: 10.1111/jpn.12877. Epub 2018 Feb 9.
The effects on rumen kinetics after feed and water had been deprived for 72 hr were studied using four fistulated Bos indicus steers. The animals were assigned in a 2 × 4 crossover design with two treatments: feed and water ad libitum (control) and no feed and water for 72 hr (deprived) with four steers per treatment over two time periods. Feed and water deprivation caused decreases in the numbers of cellulolytic bacteria (1.4 vs. 0.4 cfu × 10 /ml; p = .001), live (23.7 vs. 0.8 × 10 /ml; p = .001), dead (12.7 vs. 0.5 × 10 /ml; p = .001) and total bacterial counts (36.4 vs. 1.4 × 10 /ml; p = .001) at day 0, compared with the control treatment. However, the deprived group had greater numbers of cellulolytic bacteria (2.7 vs. 50.1 cfu × 10 /ml; p = .001), live (18.3 vs. 42.2 × 10 /ml; p = .001), dead (6. 5 vs. 19.1 × 10 /ml; p = .001) and total bacterial counts (24.8 vs. 61.3 × 10 /ml; p = .001) from rumen fluid on day 4, compared with the control treatment. The numbers of protozoa in rumen fluid from the deprived group were less than (551.2 vs. 2.4 × 10 /ml; p = .001) the control group on day 0. However, the deprived treatment had fewer protozoa in rumen fluid than the control treatment on day 4 (p = .001) and day 9 (p = .001). Volatile fatty acids and in vitro gas production as functional measurements of rumen fluid followed the same trend as the bacterial and protozoa populations. These results indicate that feed and water deprivation would have a negative but transient effect on the rumen kinetics of Bos indicus steers.
使用四头装有瘤胃瘘管的印度瘤牛阉牛，研究了禁食禁水72小时对瘤胃动力学的影响。动物采用2×4交叉设计，分为两种处理：自由采食饮水（对照）和禁食禁水72小时（剥夺组），每个处理四头阉牛，分两个时间段进行。与对照处理相比，禁食禁水导致第0天瘤胃中纤维素分解菌数量减少（1.4对0.4 cfu×10⁶/ml；p = 0.001）、活菌数量减少（23.7对0.8×10⁶/ml；p = 0.001）、死菌数量减少（12.7对0.5×10⁶/ml；p = 0.001）以及总细菌数量减少（36.4对1.4×10⁶/ml；p = 0.001）。然而，与对照处理相比，剥夺组在第4天瘤胃液中的纤维素分解菌数量更多（2.7对50.1 cfu×10⁶/ml；p = 0.001）、活菌数量更多（18.3对42.2×10⁶/ml；p = 0.001）、死菌数量更多（6.5对19.1×10⁶/ml；p = 0.001）以及总细菌数量更多（24.8对61.3×10⁶/ml；p = 0.001）。剥夺组第0天瘤胃液中原生动物数量少于对照组（551.2对2.4×10³/ml；p = 0.001）。然而，剥夺处理在第4天（p = 0.001）和第9天（p = 0.001）瘤胃液中的原生动物数量少于对照处理。挥发性脂肪酸和瘤胃液体外产气量作为瘤胃功能的测量指标，与细菌和原生动物数量的变化趋势相同。这些结果表明，禁食禁水会对印度瘤牛阉牛的瘤胃动力学产生负面但短暂的影响。
