Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India.
Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India.
Virus Res. 2018 Mar 2;247:55-60. doi: 10.1016/j.virusres.2018.01.017. Epub 2018 Feb 7.
Infectious Bursal Disease (IBD) is an acute, highly contagious and immunosuppressive disease of young chicken. The causative virus (IBDV) is a bi-segmented, double-stranded RNA virus. The virus encodes five major proteins, viral protein (VP) 1-5. VPs 1-3 have been characterized crystallographically. Albeit a rise in the number of studies reporting successful heterologous expression of VP5 in recent times, challenging the notion that rapid death of host cells overexpressing VP5 disallows obtaining sufficiently pure preparations of the protein for crystallographic studies, the structure of VP5 remains unknown and its function controversial. Our study describes the first 3D model of IBD VP5 obtained through an elaborate computational workflow. Based on the results of the study, IBD VP5 can be predicted to be a structural analog of the leucine-rich repeat (LRR) family of proteins. Functional implications arising from structural similarity of VP5 with host Toll-like receptor (Tlr) 3 also satisfy the previously reported opposing roles of the protein in first abolishing and later inducing host-cell apoptosis.
传染性法氏囊病（IBD）是一种急性、高度传染性和免疫抑制性疾病，主要发生于雏鸡。病原体（IBDV）是一种双片段、双链 RNA 病毒。该病毒编码 5 种主要蛋白，即病毒蛋白（VP）1-5。VP1-3 已通过晶体学进行了表征。尽管最近有越来越多的研究报告成功异源表达 VP5，但挑战了“过度表达 VP5 的宿主细胞快速死亡不允许获得足够纯的蛋白质用于晶体学研究”的观点，但 VP5 的结构仍然未知，其功能也存在争议。我们的研究描述了通过精心计算工作流程获得的 IBD VP5 的第一个 3D 模型。根据研究结果，IBD VP5 可以预测为富含亮氨酸重复（LRR）蛋白家族的结构类似物。VP5 与宿主 Toll 样受体（Tlr）3 的结构相似性所产生的功能影响也满足了该蛋白在最初抑制和随后诱导宿主细胞凋亡的相反作用的先前报道。
